Additional file 7 of Lactobacillus reuteri tryptophan metabolism promotes host susceptibility to CNS autoimmunity

Abstract

Additional file 7: Figure S7. The abundance of p-cresol sulfate and p-cresol glucuronide correlate with disease severity. Serum was collected from mice fed a low or high tryptophan diet colonized with either the B6 or B6+L. reuteri microbiome following a 30-day EAE course and analyzed via UPLC-MS/MS as outlined in (Fig. 4A and B). Data were analyzed for all four experimental groups: B6 or B6+L. reuteri colonized mice randomized to a low 0.02% or 0.8% high tryptophan diet. (A) Top 25 metabolites correlating with disease severity as measured by cumulative disease score (CDS), the sum of all daily scores over a 30-day disease course. (B) X-Y scatter plot of p-cresol sulfate and p-cresol glucuronide abundance and CDS using linear regression, with the P value indicating significant deviation from a non-zero slope (i.e. significant correlation). A zero value was entered for p-cresol glucuronide abundance in low-tryptophan-fed mice when below the limit of detection. Statistical analysis is provided in Table S31