Additional file 1 of Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO

Abstract

Additional file 1: Table S1. List of primers used in this study. Table S2. Sequences of genes expressed in this study. Figure S1. Image of Sanger sequencing results showed the nature of the in-frame deletion in the pta gene (CAETHG_3358). Figure S2. Image of Sanger sequencing results showed the nature of the in-frame deletion in the Ald subunit of the adhE1 gene (CAETHG_3747). Figure S3. Change in CO headspace pressure for C. autoethanogenum strains carrying plasmids for AAT expression on CO as main carbon source. Figure S4. Screening of ethyl acetate production by C. autoethanogenum strains carrying plasmids for AAT expression on 40 mM fructose. Figure S4. Investigating growth of C. autoethanogenum on CO and ethyl acetate. Figure S5. Investigating ethyl acetate degradation by C. autoethanogenum grown on CO. Figure S6. Eat1 in vivo alcoholysis assay for C. autoethanogenum and E. coli. Figure S7. Investigating the effects of ethanol supplementation on growth of C. autoethanogenum [PThl-Atf1] grown on CO. Figure S8. Investigating the effects of ethanol supplementation on ethyl acetate production by C. autoethanogenum [PThl-Atf1] grown on C