Fungal diversity and mycotoxin contamination of some selected food commodities from Ivory Coast

Abstract

Abstract: This study surveyed fungi and mycotoxins in important food crops consumed in Ivory Coast. To achieve this, the following local food items (attieke, cassava flakes, chilli, gnangnan, haricot, melon, millet, okra, rice, white maize and yellow maize) were sampled from local markets (Adjame, Cocody and Youpougon) in Abidjan, Ivory Coast. They were screened for fungal contamination based on morphological characters and confirmed by PCR using the internal transcribed spacer 1 and 4 primers (ITS1 and ITS4). A total of 227 isolates were morphologically identified withisolates dominated by species within the genera Aspegillus (54.9 %) followed by Penicillium (23.3 %) and Fusarium (14.3 %). Few isolated species were confirmed in other genera which include Alternaria, Chaetomium, Cladosporium, Epicoccum, Emerica, Rhizopus and Trichoderma spp.The highest mean fungal load of 6.85 x 105 CFU/g was found in white maize while the lowest mean level of 4.39x104CFU/g was recorded in cassava flakes. The subset of isolates were identified using ITS1 and ITS4, as Aspergillus species found to be most frequentin cassava flakes, chilli, gnangnan, haricot, rice and yellow maize. Penicillium species were also found frequent in chilli, haricot and rice, while Fusarium species were frequent in melon and millet...M.A. (Biotechnology