Aizawa, Juliana

Cavalini, Matheus Burilli

Cortez, Adriana

de Castro, Juliana Fernandes Paula

de Souza Filho, Antonio Francisco

de Souza, Gisele Oliveira

Guedes, Israel Barbosa

Heinemann, Marcos Bryan

Abstract: Changes were made to the original formulation of the EMJH medium (Ellinghausen--McCullough-Johnson-Harris) enrichment and some aspects such as growth time of Leptospira and utilization in the microscopic agglutination test (MAT) were evaluated and compared to the original enrichment and to a commercially available enrichment (DIFCOTM). Leptospira samples (24 antigens) that make up our panel of antigens used in MAT were used, among them, reference and autochthonous strains isolated in Brazil. The samples were grown individually in the EMJH medium under the three previously mentioned conditions (adapted enrichment, original enrichment and commercial enrichment). In addition, 89 blood serums from domestic and wild animals were analyzed by MAT using the antigens grown in these media. All samples tested grew efficiently with the adapted enrichment, and the MAT results were satisfactory. Therefore, other laboratories could also benefit from the use of this adapted enrichment when culturing the Leptospira strains applied in their MAT panels. (c) 2021 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/)

Institutional Repository Universiteit Antwerpen

Mejora del enriquecimiento utilizado en el medio Ellinghausen-McCullough-Johnson-Harris (EMJH) para el cultivo de Leptospira spp.

Mejora del enriquecimiento utilizado en el medio Ellinghausen-McCullough-Johnson-Harris (EMJH) para el cultivo de Leptospira spp.

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Institutional Repository Universiteit Antwerpen