This is the final report to the Environment Agency under an extension to the project, ‘Development of a phytobenthos classification tool for lakes and lochs of UK (DALES– Diatom Assessment of lake and loch ecological status)’ (contract no. SC030103), funded by the Environment Agency (EA).This project forms part of the strategy for the implementation of the European Council Water Framework Directive (WFD), which requires reference conditions to be determined for all water body types including lakes