The aim of this study was to investigate possible
differences in blood glucose levels between male and
female rats immediately after acute bout of forced
swimming exercise. Adult male Wistar rats (weight 300-
350 g) were divided into two groups by gender: males (n
=8) and females (n =8). All the rats were given standard rat
chow and tap water ad libitum and were housed at 25±3o C
on a 12-hour dark/light cycle. Both groups of rats were
exposed to forced swimming stress daily, for 6 days.
Duration of each swimming session progressively
increased from 5 minutes on the first day to 30 minutes on
sixth day, allowing adaptation to swimming conditions.
The rats were forced to swim in plastic tanks (90 cm wide,
120 cm deep) containing tap water (temperature ca. 25
degrees C). The depth of water was 40 cm. Seventh day we
performed acute bout of 40 minutes swimming exercise.
Animals were fasted 12 hours before start of last
swimming sessions to obtain fasting blood glucose levels.
Preexercise blood samples were taken immediately before
last swimming session (7th day) and postexercise samples
immediately after the last swimming session from rat\u27s tail
vein. Glucose levels in blood were determined using
Optium Xceed™ Diabetes Monitoring System (Abbot).
Before last swimming session male rats had slightly lower
glucose levels in comparation with female rats, but this
difference was not statistically significant (3.77vs4.64
mmol/l). Acute bout of forced swimming exercise raised
blood glucose level and established values in postexercise
period were significantly higher in both study group in
comparation to values before exercise. Male rats had
greater postexercise glucose blood levels (11.85 mmol/l)
in comparation with female rats (6.26 mmol/l). Our
findings document the existence of gender impact on the
glucose postexercise concentrations confirming the
differences in the energy substrates utilization and
glucose metabolism regulation during and after exercise.Cilj ove studije bio je istražiti moguće razlike razine
glukoze u krvi izmeðu muških i ženskih štakora odmah
nakon akutnog opterećenja vježbom forsiranog plivanja.:
Odraslih muški štakori soja Wistar (prosječne mase
300-350 g) su bili podijeljeni u dvije skupine po spolu:
muški (n= 8) i ženski (n = 8). Svi štakori su dobili
standardnu, komercijalnu hranu za štakore i vodu iz
slavine ad libitum, a bili su smješteni na 25 ± 3 ° C na 12-
satnom tamno / svijetlo ciklusu. Obje skupine štakora bila
su izložene vježbama forsiranog plivanja,dnevno u
trajanju od 6 dana. Trajanje svake sesije plivanja postupno
se povećavalo sa 5 minuta prvog dana do 30 minuta
šestog dana , čime se postigla prilagodba životinja na
uslove plivanja. Sedmi dan smo izveli akutno opterećenje
40 minutnog forsiranog plivanja. Štakori su plivali u
plastičnim spremnicima (90 cm širine, 120 cm dubine)
koji su sadržavali vodu iz slavine (temperature približno
25 stupnjeva C). Dubina vode iznosila je 40 cm.
Životinjama nismo davali hranu 12 sati prije početka
posljednje sesije plivanja kako bi dobili razine glukoze u
krvi natašte. Uzorci krvi za odreðivaje glukoze uzeti su
neposredno prije posljednje sesije plivanja (7.dan) i
neposredno nakon posljednje sesije plivanja iz repne vene
štakora. Razine glukoze u krvi utvrðene su pomoću
Optium ™ Xceed Dijabetes sustava praćenja (Abbot) . U
uzetim uzorcima krvi neposredno prije posljedenje sesije
plivanja utvrðene su nešto niže razine glukoze kod
muških u usporedbi s ženskim štakorima, ali ta razlika nije
bila statistički značajna (3.77vs4.64 mmol /l). Akutno
opterećenje vježbom forsiranog plivanja podiglo je
razinu glukoze u krvi i srednje vrijednosti u uzorku
uzetom neposredno nakon posljednje sesije plivanja bile
su značajno više u obje skupine u usporedbi s
vrijednostima prije vježbanja. Muški štakori imali su
značajno veću razinu glukoze u krvi (11,85mmol / L) u
usporedbi sa ženskim štakorima (6,26 mmol / L). Naši
rezultati ukazuju na uticaj spola na razinu glukoze nakon
fizičkog opterećenja potvrðujući spolne razlike u
iskorištavanju energetskog supstrata i i regulaciji
metabolizma glukoze u toku i nakon vježbanja
