Sugar fatty acid esters (SFAEs) are compounds formed by a carbohydrate linked to one or more fatty acid (FA) chains. These molecules present interesting technological properties and are commonly used as biodegradable surfactants and emulsifiers in the food, pharmaceutical and cosmetics industries [1]. Recently, the synthesis of SFAEs using enzymes as biocatalysts proved to be a greener alternative. The main problem associated with the synthesis of SFAEs is the different chemical nature of the substrates, which can significantly affect the effectiveness of the enzymatic synthesis and greatly compromise the yield of the process. Lipases are quite stable biocatalysts and have been successfully used in traditional media and alternative media containing ionic liquids (ILs) [2,3]. ILs present an interesting strategy to overcome the process limitations since they generally increase enzyme stability and can be tailored to improve the solubility of a wide range of substrates. Moreover, ILs are non-volatile, non-flammable, chemically and thermally stable and biocompatible being greener alternatives to the hazardous and volatile organic solvents commonly applied [4].
In the present work, the enzymatic synthesis of SFAEs in the commercial IL CYPHOS 104 was followed and qualitatively evaluated by thin layer chromatography. The esterification between a disaccharide and vinyl laurate was performed by immobilized Lipase B from Candida antarctica, at 60 °C, using two agitation strategies (rotatory and orbital). The results showed that SFAEs were successfully biosynthesized in the IL CYPHOS 104 using a rotatory agitation.We thank the Fundação de Amparo à Pesquisa do estado de São Paulo (process 2018/07522-6; 2014/50884-5), and Conselho Nacional de Dsenvolvimento Científico (process 301963/2017-7; 465319/2014-9).info:eu-repo/semantics/publishedVersio
