Natural products are the best source for various medicinal drugs. Regardless investigate the toxic effect of these plant extracts, the results will still unsafe and unacceptable. This study aimed to identify anti-cancer activity and cytotoxicity effect of Momordica charantia extract on different cancer cell line. Materials and Methods:to archive the aim of this study 3 different cancer cell lines (HCT116, MCF-7, and HepG2) were treated with different Momordicacharantiaextract doses (from 0-100µg) for each cell line. IC50, cell viability,apoptosis, were evaluated. Results:The effect of Momordicacharantiaextract was highly significant in HepG2 cells than HCT116 cell as well as MCF-7 which showing the IC50 of Momordicacharantia extract in HepG2 was 0.77 µg/ml while in HCT116 was 0.81µg/mland was 1.35µg/ml in MCF-7 cells respectively. Also, the effect of the Momordicacharantia extract was more potent in HCT116 compared to MCF-7 cells.Conclusions: in the light of these resultsMomordicacharantiaextract may use as anticancer pro-drugat a specific type of cancer (Liver HepG2 cell line) but still need further studies to explore the mechanism of action that led to observe different results from different cell lines despite the same extract. Keywords: Momordicacharantia, Apoptosis, MCF-7, HepG2, HCT116
