Erban, Alexander

Fischer, Axel

Giavalisco, Patrick

K Hincha, Dirk

Kopka, Joachim

Mubeen, Umarah

Pagter, Majken

Schaarschmidt, Stephanie

Sprenger, Heike

Walther, Dirk

Zuther, Ellen

English

Plants from temperate regions can be primed (cold acclimated) by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold priming has been investigated in detail, hardly anything is known about memory of a previous cold event under warm conditions and a following low temperature triggering event. We were able to show that three days of cold priming at 4°C, a seven-day lag phase at 20°C and a triggering treatment of 4°C improved the freezing tolerance of Arabidopsis Col-0 and other accessions compared to plants that were not primed before. Transcripts, metabolites and lipids as possible molecular determinants of this increase in freezing tolerance were investigated in Arabidopsis accessions Col-0 and N14 after priming, memory phase and triggering by Illumina-based RNA-Seq, GC-MS metabolite profiling and UPLC FT-MS-based lipidomics. In Col-0 and N14 93 and 128 unique differentially expressed genes, one significantly changed metabolite in N14 and three (Col-0) and six (N14) lipids could be identified comparing primed and triggered with only triggered samples with differences between differently tolerant accessions. Possible functions of these candidates will be discussed. This work identified for the first time molecular and metabolic changes accompanying cold stress memory and triggering by a second cold stress. <br/

    Aalborg UniversitetIdentification of molecular determinants of low temperature memory and triggering inArabidopsisZuther, Ellen; Schaarschmidt, Stephanie; Fischer, Axel; Erban, Alexander; Pagter, Majken;Mubeen, Umarah ; Walther, Dirk ; Giavalisco, Patrick ; Kopka, Joachim ; Sprenger, Heike; KHincha, DirkPublication date:2018Link to publication from Aalborg UniversityCitation for published version (APA):Zuther, E., Schaarschmidt, S., Fischer, A., Erban, A., Pagter, M., Mubeen, U., Walther, D., Giavalisco, P.,Kopka, J., Sprenger, H., & K Hincha, D. (2018). Identification of molecular determinants of low temperaturememory and triggering in Arabidopsis. Abstract from Second International CRC 973 Symposium , Berlin,Germany.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: August 24, 2021Identification of molecular determinants of low temperature memory and triggering in Arabidopsis  Ellen Zuther1, Stephanie Schaarschmidt1, Axel Fischer1, Alexander Erban1, Majken Pagter2, Umarah Mubeen1, Dirk Walther1, Patrick Giavalisco1,4, Joachim Kopka1, Heike Sprenger1, 3, Dirk K. Hincha1 1Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany 2 Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark  3present address: VIB-UGent Center for Plant Systems Biology, Ghent University, Technologiepark 927, 9052 Ghent, Belgium 4Max-Planck-Institut für Biologie des Alterns, Joseph-Stelzmann-Str. 9b, 50931 Köln, Germany Plants from temperate regions can be primed (cold acclimated) by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold priming has been investigated in detail, hardly anything is known about memory of a previous cold event under warm conditions and a following low temperature triggering event. We were able to show that three days of cold priming at 4°C, a seven-day lag phase at 20°C and a triggering treatment of 4°C improved the freezing tolerance of Arabidopsis Col-0 and other accessions compared to plants that were not primed before. Transcripts, metabolites and lipids as possible molecular determinants of this increase in freezing tolerance were investigated in Arabidopsis accessions Col-0 and N14 after priming, memory phase and triggering by Illumina-based RNA-Seq, GC-MS metabolite profiling and UPLC FT-MS-based lipidomics. In Col-0 and N14 93 and 128 unique differentially expressed genes, one significantly changed metabolite in N14 and three (Col-0) and six (N14) lipids could be identified comparing primed and triggered with only triggered samples with differences between differently tolerant accessions. Possible functions of these candidates will be discussed. This work identified for the first time molecular and metabolic changes accompanying cold stress memory and triggering by a second cold stress.   

Identification of molecular determinants of low temperature memory and triggering in Arabidopsis

https://vbn.aau.dk/files/288677767/Identification_of_molecular_determinants_of_low_temperature_memory_and_triggering_in_Arabidopsis.pdf

Identification of molecular determinants of low temperature memory and triggering in Arabidopsis

Abstract

Similar works

Full text

Available Versions

VBN