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Borisova, Svetlana

Draper, William

Hsiao, Victoria

Koryakina, Irina

Li, Wenzong

Yang, Yue

English

Engineering Conferences International

Engineering Conferences International ECI Digital Archives Microbial Engineering II Proceedings 4-3-2022 High-throughput enzyme engineering for commercial-scale production of natural products Irina Koryakina Yue Yang Svetlana Borisova William Draper Wenzong Li See next page for additional authors Follow this and additional works at: https://dc.engconfintl.org/microbial_ii Authors Irina Koryakina, Yue Yang, Svetlana Borisova, William Draper, Wenzong Li, and Victoria Hsiao HIGH-THROUGHPUT ENZYME ENGINEERING FOR COMMERCIAL-SCALE PRODUCTION OF NATURAL PRODUCTS   Irina Koryakina, Amyris, Inc. , USA koryakina@amyris.com Yue Yang, Amyris, Inc. , USA Svetlana Borisova, Amyris, Inc. , USA Victoria Hsiao, Amyris, Inc. , USA William Draper, Amyris, Inc. , USA Wenzong Li, Amyris, Inc. , USA   Key Words: enzyme engineering, natural products, high-throughput   The natural products world is unparalleled in its molecular diversity and wide application space. There are, however, numerous challenges associated with realizing the full commercial potential of these molecules. Amyris has fermentatively produced nine different molecules at commercial scale in the past ten years. This track record is due to investment in advanced tools for strain engineering, high throughput screening, analytics, and bioinformatics. An integrated pipeline encompassing these tools has enabled Amyris to rapidly accelerate the engineering cycle and reduce the number of design-build-test iterations needed for microbial production of any natural product. In this presentation, we will discuss how this infrastructure is now being leveraged for high-throughput enzyme mutagenesis and screening, enabling greater access to natural products and their derivatives. Further, the application of our massive screening infrastructure to enzyme libraries would not be possible without equally sophisticated statistical models and data analysis tools. Scientists at Amyris are accessing ever greater portions of the enzyme sequence space to improve specificity and activity – ultimately enabling sustainable industrial-scale production of natural products. This talk will describe how each aspect of the enzyme engineering pipeline has led to rapid and high-quality screening of hundreds of thousands of mutants for multiple enzymes.     

High-throughput enzyme engineering for commercial-scale production of natural products

https://dc.engconfintl.org/cgi/viewcontent.cgi?article=1062&context=microbial_ii

High-throughput enzyme engineering for commercial-scale production of natural products

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Engineering Conferences International