Extraction, Purification, and Characterization of Bioactive Compounds Produced by Janthinobacterium Lividum Strain TAJX1901

Abstract

Unexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce potent secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing novel natural compounds. Secondary metabolites isolated from microorganisms are currently being used in the production of several antibiotics. The current research primarily seeks to perform bioactivity-guided isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum strain TAJX1901). To achieve this objective, liquid-liquid extraction of a rich medium (RM) agar inoculated with the soil bacterium was performed using a solvent mixture of chloroform and methanol (1:3). The agar extract was fractionated by flash column chromatography using a gradient elution of ethyl acetate and petroleum ether. For purification purposes, the crude fractions were subjected to both analytical and preparative thin-layer chromatography with a further option of high-performance liquid chromatography (HPLC). We also intend to use spectroscopic techniques like two-dimensional (2D) nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) to investigate the chemical structure of the extracted pure compounds