Aso, Yoichi

Funatsu, Gunki

Ishiguro, Masatsune

Ohnuma, Takayuki

Tanigawa, Miho

Tsutsumi, Kazuki

Yamagami, Takeshi

amino acids with a calculated molecular mass of 67kDa. The deduced amino acid sequence of T. halophila DnaK showed high similarities with the corresponding DnaK homologues of Lactococcus lactis, Lactobacillus sakei and Bacillus subtilis. Using a pET expression system, the T. halophila DnaK was overexpressed in Escherichia coli and the purified T.halophila DnaK was found to have ATPase activity. Northern hybridization analysis revealed that the transcription of dnaK gene was induceed by heat shock, and several transcripts were detected including a maximum size of tetra-cistronic mRNA 4.9-kb which represents the transcript complete dnaK operon. The amount of dnaK transcripts was also increased about 3.5-fold by high NaCl condition （3-4 M）, but not by the same concentration of KCI. These results suggest that the cloned DnaK surely acts as the functional molecular chaperone and play an important role in the salinity adaptation

石黒, 正恒

山上, 健

船津, 軍喜

大沼, 貴之

麻生, 陽一

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Modification of Tryptophan Residues of Plant Class III Chitinases Involved in Enzyme Activity

Modification of Tryptophan Residues of Plant Class III Chitinases Involved in Enzyme Activity

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