Bungo, Takashi

Goto, Ichiro

Kawamura, Toshihisa

Masuda, Yasuhisa

Shimojo, Masataka

Streptomyceslaurentii ATCC 31255, wild-type strain PO, formed spontaneously developing pocks with the production of phage taillike particles. Electron micrographs showed that much hyphae in the pocks were broken or lysed. Around the broken hyphae, there were many hexagonal ringlike particles and some kinds of tubelikc particles. A large (SO-90 kbp) plasmid designated as pSLL was isolated from strain PO. Another covalently closed circular plasmid, pSLS(16 kbp), was found in pSLL-cured PO cells (named P1), which formed spontaneously pocks on a solid medium. The frequency of the generation of pSLL-loss P1 cells coordinated positively with the frequency of pock appearance. Thiostrepton productivity of strain Pl showed a decrease of about 50%) compared with that of wild-type strain PO. The pSLS was also found as an integrated form in the chromosome of strains PO and P1. The results indicated that. strain PO harbored two plasmids, pSLL and integrated form pSLS, and pSLS seemed to be generated as a free-form plasmid with the loss of pSLL. It was also suggested that, pSLL may suppress the excision of the integrated pSLS from the chromosome and that the free form of pSLS may participate in pock formation

下條, 雅敬

増田, 泰久

豊後, 貴嗣

河村, 俊久

五斗, 一郎

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Analytical Expression for Formation of Indigestible Materials and Increase in Dry Matter Indigestibility with Growth of Some Tropical Grasses

Analytical Expression for Formation of Indigestible Materials and Increase in Dry Matter Indigestibility with Growth of Some Tropical Grasses

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