Hatano, Shoji

Honjoh, Ken-ichi

Iio, Masayoshi

Maruyama, Isao

Miyamoto, Takahisa

Shinohara, Fuminori

Suga, Koushirou

Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of naked protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73112 cells were covereted into osmotically-labile cells. Futhermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5M sucrose as an osmoticum, Fe-EDITA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73112 and C-27

本城, 賢一

ホンジョウ, ケンイチ

菅, 向志郎

スガ, コウシロウ

篠原, 史訓

シノハラ, フミノリ

丸山, 功

マルヤマ, イサオ

宮本, 敬久

ミヤモト, タカヒサ

波多野, 昌二

ハタノ, ショウジ

飯尾, 雅嘉

イイオ, マサヨシ

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Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27

Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27

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