Louwerens, Bernardus

University of Groningen

   University of GroningenFoa-Kurloff cellen. De invloed van enige hormonen, in het bijzonder geslachtshormonen, ophet aantal Foa-Kurloff cellen in het bloed van de caviaLouwerens, BernardusIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1952Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Louwerens, B. (1952). Foa-Kurloff cellen. De invloed van enige hormonen, in het bijzondergeslachtshormonen, op het aantal Foa-Kurloff cellen in het bloed van de cavia. Groningen: s.n.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 12-11-2019rum, Íntraveneus ingespo-.4, het aantal granulocy-van een cavia met weinignbl Ík  van b loedafnemen,en neiging tot dalen van: hebbenij vaststellen:Itstreekse invloed op het;ecundaire invloed op hethet ovarium tot vergrote;, hebben geen invloed.l .  F.K.cel len wordt  w&&r-de oestradiol concentra-c blj de mlnlmun dosís.op over de functle vanrrtbestaan van de F.K.ceIn het onderzoek zou kun-S U M M A  R YIn the b lood of  the guinea p ig a ceI I  is  found that  can mosteasi ly  be descr ibed as a b ig lymphocyte wi th one,  somet imes twovacuoles. In the bloodsmear, stained according to May Griinwald-GÍemsa, this vacuole appears to be fi l led with fi laments or gra-nules consisting of a substance which is stained in about the sameway as the nucleus. After the investigators who were the first toreport on these cells, they are called Fbà-Kuloff cel1s (abbrevi-a ted  to  F .K .ce1 l s ) .A number of hypotheses have been put forward on the origin andnature of these cells. The most probable one is based on the im-por tant  fact  that  the F.K.cel Is  increase af ter  admlnis t rat ion ofoestradio l  and that  testosterone is  supposed to have a s imi laref fect .The purpose of our investigatlon was to obtain more informa-t ion about  the act ion of  sex hormones on the F.K.ce1ls in  maleand fenale guinea pigs. Inaddition by removal of endocrine glandsor by administering other hormones we tried to establish if otherendocr ine.organs but  sex organs af fect  the number of  F.K.ceI ls .Since there is no agreement about this we finally tried to esta-b t i sh  i f  a  co r re la t i on  ex i s ted  be tween  the  number  o f  F .K .ce l l sand the nrmber of any other cells of the white blood picture.ïhe substances were always administered subcutaneously. Begin-nÍng with the day of lnjection the blood ce1ls were counted everytwo days (our schedule was: blood sampling on l, londag \ïednesdayand Friday, or Tuesday, Thursday and Saturday in the afternoon).We counted the total number of the white cells and differentiatedinto lymphocytes, eosinophil-e granulocytes, other granulocytes andF.  K.  ce l  1s.Our results, also mentioned at the end of the chapters, were:Chapter IV.1.One iq 'ect ion of  oestradio l  causesapeak in the number of  c ' .K.cells 8 to 14 days after the administration Whereas in normalguinea pigs about 3 per cent of the white bloodcells appear tobe F.K.  ceI ls ,  th is  percentage increases t i11 8 to 10 per  centafter the administration of oestradiol. There also occur peaksfrom 16 to 20 per cent, but these were exceptions.. In the maleguinea pig the normal nunber of 1 to 2 per cent F.K. cells in-creases af ter  adminis t rat ion of  oestradio l  t i l l  8  to  10 percent in about ten days.2. In the normal female the smallest effective dose of oestradioll ies between 1 y and 2.5 y oestradiol per 100 g of bodyweight.3. Higher dosages do not have a greater effect.8 ?4. Bepeated administration of oestradiol does not change the ef-fect mentioned under 1.5. l.lormal male animals respond to an administration of oestradiolroughly in the same way as female animals, but the effect onthe p'.K.ce1Is is of longer duration: whereas in female animalsafter the highest peak the normal level of the p.K. cells re-turned in about five days, in male animals it renained hj.gh forsone weeks after the peak.6. In the normal fenale guinea pig testosterone causes a rise inthe number of F.K. cells of the same nature as that caused byoestradiol. The effect, however, is less pronounced.7. Progesterone appeared to have no effect on the number of F.K.cells in the normal female guinea pig;Chapter V,8. In the male animal castration has no effect on the number ofF .K .  ce11s .9. Castration does not change the effect of oestradiol.10.The number of  F.K.  ce l ls  in  the female animal  decreases af terovariectomy. We failed to observe the temporary increase of thelevel  of  the t r .K.  ce l ls  immediate ly  af ter  the operat Íon whichis reported by others.11.oestradio,l raises the number of F.K. cells in the ovariectomizedguinea pig, but the effect is less pronounced as the time be-tween the operation and the injection of oestradiol is longer.l2.Subcutaneousimplantation of two tablets of 25 mg of oestradiolberzoate in ovariectomized animals caused a peak of the numberof  F.K.ce11s,  which is  s imi lar  to  the peak af ter  one in ject ionof oestradiol in the nornal female. After the peak the levelof  the F. !L cel ls  remains increased for  some t ime.  Af ter  re-moval of the implant the nunber of F.K. cells decreased. Thisdecrease is not preceded by an increase (compare 10).12. In dosages up to 0.5 ng per  100 g of  bodyweight  testosteronehas no ef fect  on the number of  F.K-  cel ls  inovar iectomizedguinea p igs.13. In ovar iectomized animals,  which level  of  the F.K.  ce l ls  wasmaintained on about 3 per cent of the number of white blood-cells by means of an oestradiol benzoate implant, testosteroneproved to have no effect on the number of F.l(. cells.Chopter  Y) .l5.Neither adreno-corticotropic hormone (AC1Ïl) nor desoxycortico-steronacetate (DcA) were able to affect the number of clrcul-ating F.I(. cells in the normal female guinea pig.16.Rènroval of the adrenal glands did not change the numberofF.K.ce1ls in the female guinea pig.lT.Hypophysectomized females respond to one injection of oestra-88dio lder  118. Afterthe b19. Adnln,cel ls20. Afterhad aChapter I21. Togetlalwayssequela decrmean trlse 1Clwpter I22.No corother23. Intravplg wlper cefemalewith frcrease0n theí .Oes t ra r2. Testos'F.K.ce,of  csJ. As far4. Tlte lnprobabF.K.  cr5. lhe optdose.FinalIoestradiofew sugge)es not change the ef-stration of oestradiol, ls, but the effect onreas in female animalso f  t he  F .K .  ce l l s  re -s it reneined high forSrone causes a rise inbure as that caused by, pronounced.on the number of F. K.lfect on the number ofI oestradiol.animal decreases afterrpo{ary increase of theer the operation whichLs in the ovariectomizedounced as the time be-' oestradiol is longer.of 25 mg of oestradiolI a peak of the numberak after one injection;er the peak the level' sonp time. After re-cel ls  decreased.  This(compare 10).dWeight testosteronel ls  in  ovar iectomizedl f  t he  F .K .  ce l l s  wasrmber of white blood-implant" testosteroneI  F. l ( .  ce l ls .t l) nor desoxycortico-lhe number of clrcul-nea pig.nge the number of F. K.injection of oestra-diol with a rise of the number of F.K. cells, as mentioned un-de r  1 .18.After hwophysectomy the F.K ce1ls gradually disappear fromthe blood.19.Admlnistration of adrenaline does not change the numberofF.K.cells ln normal femles.20.After renoval of the uterus (1n only two anlmals) oestradiolhad a prolonged action.Chapter VII.2 l .Together  wi th the increase of  the number of  F.K.  ce l ls  therealways occurs a rise ln the nrnnber of granulocytes and the sub-sequent decrease of the F.K. cells is l lkewlse accompanied bya decrease of the granulocytes. On the contrary thls does notmean that the number of F.lG cells increases when there is arlse ln the granulocytes caused by some other reasonChopter VIII.22.No correlation ïras found between the number of F.lL cêlls andother cells of the whlte blood plcture.23.Intravenous administration of 2 ml serum obtained fromaguineapig wlth a number of F.K. cells in the blood of more than 10per cent, lncreased the number of granulocytes of the normalfenale guinea pig. Onthe contrary serum obtained fromananimalwith few F.I(. cells in the blood caused a tendency towards de-crease of the nunber of granulocytes.0n the basls of these data it is concluded:í. Oestradlol has a dlrect effect on the number of F.K. cells.2. Testosterone probably has an indirect effect on the number ofF.K.celIs. This honpne is supposed to stlmulate the secretionof oestradlol by the ovary.J- As far as investigated other hompnes have no effect.4. Ttie lncrease of the oestradlol concentration of the blood isprobably the stimulus whlch caused the rise of the number ofF.K.  ce l ls .5. the optimal dose of oestradiol is nearly the same as the minimaldose.Finally a hypothesis was put forward about the importance ofoestradiol to the origin and maintenance of the F.K. cells and afew suggestions were given for continuing the investigations.89

Foa-Kurloff cellen. De invloed van enige hormonen, in het bijzonder geslachtshormonen, op het aantal Foa-Kurloff cellen in het bloed van de cavia

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Foa-Kurloff cellen. De invloed van enige hormonen, in het bijzonder geslachtshormonen, op het aantal Foa-Kurloff cellen in het bloed van de cavia

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