The main objectives of this study were to assess current gender mainstreaming practices and to identify opportunities and challenges in mainstreaming of gender issues in selected government offices in Fogera district, Ethiopia. The specific objectives of the study were to assess and analyze the practice of gender mainstreaming, to identify the existing opportunities for gender mainstreaming and to examine the major practical problems that inhibit gender mainstreaming. The study used qualitative research methods and case study research design. Consequently, purposive sampling was used to select the informants and discussants of the study. The findings showed that most participants of the study who are currently working in study offices are clear with the concepts of gender and gender mainstreaming, but they did not have clear understanding on the point that promoting gender equality. Besides, the study offices did not fully employ different tools and techniques for gender mainstreaming practice. The finding also showed that the participants of the study offices have less access to training and retraining opportunities of gender and gender mainstreaming. Lack of commitment and attention on the part of decision-making bodies, lack of continuous access to gender awareness and sensitivity training were identified as the major challenges