Carvalho, Nelson Mota de

Costa, Célia Maria

Madureira, Ana Raquel

Oliveira, Diana Luazi

Pintado, Manuela Estevez

English

Repositório Institucional da Universidade Católica Portuguesa

Human and animal in vitro gastrointestinal models: importance and applications Nelson Mota de Carvalhoa, Diana Luazi Oliveirab, Célia Maria Costaa, ManuelaEstevez Pintadoa and Ana Raquel Madureiraaa Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superiorde Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugalb Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho, 1327, 4169-005 Porto, PortugalConclusionsIn vitro models can provide valuable information on functional ingredient suitability for the food/feed matrix inquestion, according to the host needs, contributing greatly for the development of new ingredients and minimizingthe number of in vivo studies.In vitro gastrointestinal models can be used to screen food and feed ingredients by simulating the hostphysiological parameters, enabling the understanding of how different ingredients modulate the gut microbiotaand the production of metabolites.Functions of gut microbiota• Fermentation of undigested food/feed• Short chain fatty acids (SCFA) production • Protection against pathogens or non-indigenous microorganisms• Detoxification and stimulation of the immune systemFunctional ingredients properties• Generally recognized as safe (GRAS)• No contaminants and impurities• No alteration of gut microbiota balance• Non-carcinogenic• Heat and pH stability• Techno-functional properties (e.g., sweetener, thickener, etc.)• Additional nutrients supplyKey factors• Inoculum• pH• Temperature• Fermentation time• Food/ Feed matrixAdvantages• Simple• Flexible• Reproducible• Less time• CheaperIn vitro modelsBenefits related to SCFA• Host energy and hydration• Bacteriostatic properties• Maintenance of the gut barrier function• Increase of the gastrointestinal tract absorption surface• Anti-inflammatory role in gut mucosa• Satiety and reduction of food intakeThis research was funded by Amyris Bio Products Portugal Unipessoal Lda and Escola Superior de Biotecnologia – Universidade Católica Portuguesa throughAlchemy project - Capturing high value from industrial fermentation bio products (POCI−01−0247−FEDER−027578). We would also like to thank the scientificcollaboration under the Fundação para a Ciência e a Tecnologia (FCT) project UIDB/50016/2020.AcknowledgementsGut microbiota has a key role and major impact on the host’s health. Therefore, the need to maintain the gutmicrobiome community balanced and healthier has driven to an increase introduction of functional ingredients inthe diet of both human and animals.BackgroundAssessment parameters• Metabolites productionExamples: lactate, acetate, propionate, butyrate andvalerate• Ammonia/ammonium concentration (NH3 / NH4+)• Gut bacteria quantification and viabilityExamples: Lactobacillus, Bifidobacterium, Clostridium,Bacteroides and Enterobacteriaceae

Human and animal in vitro gastrointestinal models: importance and applications

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Human and animal in vitro gastrointestinal models: importance and applications

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Repositório Institucional da Universidade Católica Portuguesa