While age of Information (AoI) has gained importance as a metric characterizing the freshness of information in information-update systems and time-critical applications, most previous studies on AoI have been theoretical. In this chapter, we compile a set of recent works reporting AoI measurements in real-life networks and experimental testbeds, and investigating practical issues such as synchronization, the role of various transport layer protocols, congestion control mechanisms, application of machine learning for adaptation to network conditions, and device related bottlenecks such as limited processing power