Background: In the recent past, many studies have been focused on
extracts of BF and multiple biologically active factors and their
effects on humoral immune system in chickens and birds. However, the
mechanism of those immunomodulatory peptides on the B lineage cells
proliferation and antibody production in chicken is fairly unknown.
DT40 cell line, an avian leucosis virus-induced chicken pre-B cell
line, expresses immunoglobulin M (IgM) isotype B cell reporter in the
plasma membrane. There are many evidences suggesting that DT40 cells
are best characterized as a bursal stem cell line. Because of the
unique characteristics of DT40 cell line, it has been widely used to
observe biological processes of pre-B lymphocyte cell within living
cells. Methods: The chicken B cell line DT40 was cultured in Roswell
Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was
studied. Also, effect of BP5 on cell proliferation and cell cycle
distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM
mRNA expression was studied by using real-time PCR. Objectives: To
investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a
chicken promyelocyte cell line DT40, assays of cell proliferation, cell
cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA
expression and gene microarray analysis were performed. Results: The
results showed that BP5 displayed concentration-dependent effects on
the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells.
And the analysis of expression profiles identified a signature set of
3022 genes (1254 up regulated genes, 1762 down regulated genes), which
clearly discriminated the BP5-treated DT40 cells from control with high
certainty (P 640.02). The results of microarray analysis were
confirmed by quantitative reverse transcription-polymerase chain
reaction for 12 of the differentially expressed genes. Conclusion:
Theses findings showed the immuno-activity effect of BP5 on B
lymphocyte and indicated that BP5 treatment regulated eight signaling
pathways, in which Toll-like signaling pathway was the most significant
enrichment pathway