Background: Avian influence virus H5N1 causes serious public health
concern with significant morbidity and mortality from poultry to
humans. Interferon-induced transmembrane (IFITM) proteins usually
protect cells from many virus infections by viral entry and
replication. Objectives: The purpose of this study was to investigate
whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and
IFITM3 following H5N1 infection. Methods: NS1, M1, NP, PB2, HA and NA
genes of H5N1 virus were generated by PCR and cloned into
pcDNA3.1/myc-His (+) A vector for genes over-expression experiments.
Gene expression levels was performed using Real-time PCR. Results:
Research displayed that NS1, M1, NP, and PB2 proteins of H5N1 virus
increased IFITM1, IFITM2, and IFITM3 expression in A549 cells, only
IFITM1 was upregulated by M1 in HEK293T cells. However, our study did
not find that HA and NA of H5N1 virus affected IFITM genes family or
interferon genes expression. Conclusion: Taken together, our data
suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated
via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus
infection. This study provided new insights into the influence of NS1
and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression
following H5N1 infection. DOI: https://dx.doi.org/10.4314/ahs.v19i1.13
Cite as: Wang H, Chen L, Luo J, H H. NP and NS1 proteins of H5N1 virus
significantly upregulated IFITM1, IFITM2, and IFITM3 in A549 cells.
Afri Health Sci. 2019;19(1). 1402-1410.
https://dx.doi.org/10.4314/ahs.v19i1.1