Enhancement of botrallin and TMC-264 production in liquid culture of
endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments
with metal ions
Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from
a poplar hybrid, was a high producer of botrallin and TMC-264 with
various bioactivities. In this study, the influences of eight metal
ions (i.e., Mn2+, Na+, Mg2+, Zn2+, Cu2+, Fe2+, Fe3+ and Al3+) on
botrallin and TMC-264 production in liquid culture of the endophytic
fungus Hyalodendriella sp. Ponipodef12 were investigated. Results:
Three most effective metal ions (Zn2+, Cu2+ and Mg2+) along with their
optimum concentrations were screened. The optimum addition time and
concentrations of Zn2+, Cu2+ and Mg2+ were further obtained
respectively for improving botrallin and TMC-264 production. The
combination effects of Zn2+, Cu2+ and Mg2+ on the production of
botrallin and TMC-264 by employing statistical method based on the
central composite design (CCD) and response surface methodology (RSM)
were evaluated, and two quadratic predictive models were developed for
botrallin and TMC-264 production. The yields of botrallin and TMC-264,
which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were
validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum
concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and Mg2+ at
0.13 mmol/L in medium. Conclusion: The results indicated that the
enhancement of botrallin and TMC-264 accumulation in liquid culture of
the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions
and their combination should be an effective strategy