Greenhouse evaluation on the performance of heat tolerant transgenic
broccoli and genetic diversity analysis using inter simple sequence
repeat (ISSR) markers
Background: Broccoli, Brassica oleracea subsp. italica is one of the
many valuable Brassica species which is still less cultured under in
vitro condition. Heat tolerant transgenic and non-transgenic broccoli
cv. Green Marvel plantlets with well-developed root system obtained
through in vitro culture were transferred into disposable plastic pots
containing sterilized potting mixture consisting of (peatgroTM) +
coconut dust (2:1) and maintained in a growth chamber. Results: After
one month, the hardened plantlets were transferred and maintained in a
transgenic greenhouse. After four months of acclimatization in the
transgenic greenhouse, the efficacy of HSP101 gene in increasing the
heat tolerance of the transgenic broccoli was evaluated. Results showed
that the transgenic plants could survive and performed normally,
producing flower heads even at the highest tested temperature of
34\ubaC. Seven transgenic broccoli lines with different gene copy
number of the AtHSP101 gene as well as the control plant were assessed
for genetic diversity using inter simple sequence repeat (ISSR)
markers. Conclusions: ISSR results showed polymorphism and phylogenetic
relationship between the transgenic and non-transgenic (control)
Brassica oleracea cv. Green Marvel