Background: Based on the conserved sequences of a known NBS resistance
gene, a pair of degenerate primers was designed to amplify the NBS-LRR
resistance gene from peanut using PCR and RACE methods. Results:
Analyzing the amino acid sequence by BLAST on NCBI, which was deduced
from the 1088bp-long gene named PnAG1-2, showed that it had a certain
homology with some resistance proteins, among which Arachis cardenasii
resistance protein gene had the highest homology (66%). Relative
quantification PCR analysis indicated that PnAG1-2 gene expresses more
in J11 (an A. flavus-resistant variety) than in JH1012 (an A.
flavus-susceptible variety) when the harvest time was coming.
Conclusions: In this study, the NBS-LRR resistance sequence was
successfully cloned from peanut and prokaryotic expression was done on
the gene, which provided a foundation for cultivating anti-A. flavus
peanut varieties