S-Adenosyl Methionine (SAMe) Synthetase is an enzyme which catalyses
the synthesis of S-Adenosyl Methionine using methionine and ATP. It is
also known as AdoMet which is well known methyl donor, which modifies
DNA, RNA, histones and other proteins, dictating replicational,
transcriptional and translational fidelity, mismatch repair, chromatin
modeling, epigenetic modifications and imprinting. The objective of the
present work is to clone the SAMe Synthetase gene in recombinant E.
coli strain in order to express, characterize and purify it for further
synthesis of SAMe in a large scale fermentation. Expression was induced
by 1 mM IPTG and expressed protein was characterized by SDS-PAGE. The
recombinant E. coli cells were used for the production of SAMe through
batch and fed batch fermentation operations. The produced SAMe was
purified through paper chromatography in order to use it in our future
studies