For biotechnological purposes, protein expression refers to the
directed synthesis of large amounts of desired proteins. The aim of the
present work was to produce reverse transcriptase Moloney murine
Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as
bioactive products. In the present paper, we report the preparation of
recombinant enzymes, expressed in E. coli strains. The enzymes produced
exhibited quite good activity, compared with commercial enzymes,
allowing us to replace the last ones for several lab applications. We
are reporting changes and modifications to standard protocols
described. The standard protocols were modified, i.e. for the
purification step of Taq, a temperature dependent procedure was
designed. The enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs molecular markers