Eucalyptus globulus is the most important commercial temperate
hardwood in the world because of its wood properties and due to its
characteristics for biofuel production. However, only a very low number
of expressed sequence tags (ESTs) are publicly available for this tree
species. We constructed a cDNA from E. globulus seedlings subjected to
low temperature and sequenced 9,913 randomly selected clones,
generating 8,737 curated ESTs. The assembly produced 1,062 contigs and
3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX
analysis, 89.3% of the contigs and 88.5% of the singletons had
significant similarity to known genes in the non-redundant database of
GenBank. The Eucalyptus unigene set generated is a valuable public
resource that provides an initial model for genes and regulatory
pathways involved in cell wall biosynthesis at low temperature