In vitro suppression of pathogenic fungi by Streptomyces spp.

Abstract

The use of living organisms or natural enemies of pathogens to control their populations is called biological disease control. It involves harnessing and introduction of exotic species of microorganism in a natural form, with the intention of controlling pathogens that may exist naturally in the same ecosystem. Prospects for biological control of Aspergillus flavus , Fusarium oxysporum and Penicillium italicum were investigated using Streptomyces spp. isolated from Chinhoyi University of Technology Farm soils in Mashonaland West, Zimbabwe. Twenty seven Streptomyces spp were obtained from the soil, and screened for antimicrobial activity and antagonism in in vitro pathogen inhibition assays, replicated thrice. Although majority of the isolates tested elicited no effect on test pathogens, 22% of the Streptomyces isolates were able to effectively suppress A. flavus, F. oxysporum and P. italicum by at least 55%. There was a significant interaction between Streptomyces isolates and pathogen (A. flavus, F. oxysporum and P. italicum) (P<0.05) on fungal radial growth at days 7, 10 and 14 after pathogen-Streptomyces incubation. Antimicrobial potential against individual and multiple test pathogens was observed, with CUT-Streptomyces 4, CUT-Streptomyces 10, CUT-Streptomyces 11, CUT-Streptomyces 20 and CUT-Streptomyces 23 showing the greatest antimicrobial activity. CUT-Streptomyces isolates have the potential to suppress A. flavus, F. oxysporum and P. italicum in vitro.L\u2019utilisation d\u2019organismes vivants ou d\u2019ennemis naturels d\u2019agents pathog\ue8nes pour contr\uf4ler leurs populations est appel\ue9e contr\uf4le biologique des maladies. Il s\u2019agit d\u2019exploiter et d\u2019introduire des esp\ue8ces exotiques de microorganismes sous une forme naturelle, dans le but de contr\uf4ler les agents pathog\ue8nes pouvant exister naturellement dans le m\ueame \ue9cosyst\ue8me. Les perspectives de lutte biologique contre Aspergillus flavus , Fusarium oxysporum et Penicillium italicum ont \ue9t\ue9 \ue9tudi\ue9es \ue0 l\u2019aide de Streptomyces spp. isol\ue9es des sols agricoles de l\u2019Universit\ue9 de Technologie de Chinhoyi \ue0 Mashonaland West, Zimbabwe. Vingt-sept Streptomyces spp ont \ue9t\ue9 obtenus du sol et cribl\ue9s pour l\u2019activit\ue9 antimicrobienne et l\u2019antagonisme dans des tests d\u2019inhibition des agents pathog\ue8nes in vitro, r\ue9pliqu\ue9s trois fois. Bien que la majorit\ue9 des isolats test\ue9s ne provoquent aucun effet sur les agents pathog\ue8nes test\ue9s, 22% des isolats de Streptomyces sont capables de supprimer efficacement au moins 55% des A. flavus, F. oxysporum et P. italicum. Il y avait une interaction significative entre les isolats de Streptomyces et l\u2019agent pathog\ue8ne (A. flavus, F. oxysporum et P. italicum) (P< 0.05) lors de la croissance radiale des champignons aux 7\ue8me, 10\ue8me et 14\ue8me jours apr\ue8s l\u2019incubation de l\u2019agent pathog\ue8ne-Streptomyces. Un potentiel antimicrobien contre des agents pathog\ue8nes individuels et multiples a \ue9t\ue9 observ\ue9. CUT-Streptomyces 4, CUT-Streptomyces 10, CUT-Streptomyces 11, CUT-Streptomyces 20 et CUT-Streptomyces 23 ont montr\ue9 l\u2019activit\ue9 antimicrobienne la plus \ue9lev\ue9e. Les isolats de CUT-Streptomyces ont le potentiel de supprimer A. flavus, F. oxysporum et P. italicum in vitro