IN VITRO GERMINATION AND PROPAGATION OF CEREJEIRA ( Amburana acreana (Ducke) A.C. Smith - FABACEAE)

Abstract

A cerejeira ( Amburana acreana (Ducke) A.C. Smith) \ue9 uma esp\ue9cie arb\uf3rea nativa da Amaz\uf4nia Sul- Ocidental de grande import\ue2ncia madeireira, no entanto, encontra-se amea\ue7ada de extin\ue7\ue3o. A propaga\ue7\ue3o por cultura de tecidos desta esp\ue9cie \ue9 de grande import\ue2ncia devido \ue0s dificuldades de coleta de sementes. Os objetivos deste trabalho foram avaliar a germina\ue7\ue3o in vitro e estabelecer protocolos para multiplica\ue7\ue3o e enraizamento in vitro de brotos de cerejeira. Para a germina\ue7\ue3o in vitro foram avaliadas diferentes concentra\ue7\uf5es salinas do meio MS e WPM (Woody Plant Medium). Foram utilizados segmentos nodais de pl\ue2ntulas germinadas in vitro para a multiplica\ue7\ue3o de brotos, onde se testaram diferentes concentra\ue7\uf5es de BAP (0; 0,25; 0,5; 1,0; 2,0; 4,0; 8,0 mg.L-1) combinadas com ANA (0,1 mg.L-1). O enraizamento in vitro foi induzido com meio WPM/2, acrescido de 0,2 mg.L-1 de carv\ue3o ativado, suplementado com diferentes concentra\ue7\uf5es de AIB (0; 0,5; 1,0; 2,0 mg.L-1). O maior percentual de germina\ue7\ue3o in vitro foi obtido com o meio MS/2. A forma\ue7\ue3o do maior n\ufamero de brotos ocorreu com a concentra\ue7\ue3o de BAP de 4,0 mg.L-1. A forma\ue7\ue3o do maior n\ufamero de ra\uedzes ocorreu com 0,5 mg.L-1 de AIB. As pl\ue2ntulas foram aclimatizadas em casa de vegeta\ue7\ue3o com percentual de 54% de sobreviv\ueancia. A propaga\ue7\ue3o in vitro da cerejeira \ue9 uma tecnologia vi\ue1vel para a produ\ue7\ue3o de mudas apesar das dificuldades na sobreviv\ueancia de plantas em casa de vegeta\ue7\ue3o.Cerejeira ( Amburana acreana ) is a woody native tree species from south-western Amazon Region of a great importance and endangered. The propagation by tissue culture of this species is of a great importance because the difficulties of its seed collection. The purpose of this study was to evaluate the in vitro germination and to establish a protocol for in vitro multiplication and rooting. For in vitro germination, the salt concentrations of MS and WPM (Woody Plant Medium) were evaluated. For in vitro multiplication of shoots it was used nodal segments of in vitro seedlings. The propagation media were composed of WPM salts, supplemented with different BAP concentrations (0, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0 mg.L-1) and 0.1 mg L-1 of NAA. The in vitro rooting was induced in half strength WPM salts, plus 0.2 mg L-1 activated charcoal and supplemented with different IBA concentrations (0, 0.5, 1.0, 2.0 mg.L- 1). The highest percentage of in vitro germination was obtained in the MS/2 medium. The formation of largest number of shoots occurred with 4.0 mg L-1 BAP. The formation of a greater number of roots occurred with 0.5 mg L-1 of IBA. The plantlets were air-conditioned in a greenhouse with 54% of survival rate. The in vitro propagation of Cerejeira is a viable technology for the production of seedlings in spite of the difficulties in the survival of plants in the greenhouse