The article presents research on the exploring of extraction process of biologically active substances from the leaves
of the Moringa oleifera tree using ethanol. Ethanolic extracts were obtained using three different techniques: maceration with shaking, ultrasound-assisted extraction and extraction in Soxhlet apparatus, in different time variants:
1, 2 and 4-hours. After solvent evaporating and drying, the yields of dry extracts obtained in particular processes
were calculated. The antioxidant activity of extracts was analyzed spectrophotometrically using DPPH radical
scavenging method, and total phenolic content (TPC) was determined by Folin-Ciocalteu method. By means of
gas chromatography with mass selective detector (GC-MS), 11 biologically active compounds present in ethanolic
extracts were identified, among which α-tocopherol had the greatest share. Based on the results, the influence of
the extraction technique and time on the yield and antioxidant activity of M. oleifera leaf extracts were discussed