27 páginas, 4 figuras, 4 tablas.Gelatin extracted from tuna skins and giant squid tunics
were hydrolysed with Alcalase at 50ºC for 3h. Two peptide
fractions (1-10K and ≤1K) were obtained from each gelatin
hydrolysate by subjecting them to centrifugal ultrafiltration using
successively a 10 kDa and a 1 kDa membrane. The peptide
fractions were characterized in terms of amino acid composition
and Fourier transform infrared (FTIR) spectroscopy. Antioxidant
properties were tested according to the Ferric Reducing
Antioxidant Power (FRAP) assay and the radical scavenging
capacity (ABTS) assay. A disk diffusion test was performed to test
antimicrobial action against a panel of Gram-positive and Gramnegative
pathogenic and fish spoilage-associated microorganisms.
Although antioxidant and antimicrobial properties could be
detected in all tested peptide fractions, the lowermost molecular
weight fraction from squid hydrolysate presented the highest
reducing and radical scavenging capacities, whereas microbial
growth inhibition was found to be specifically related to the type of
microorganism.This study was supported by the Spanish “Ministerio de Educación y
Ciencia” (project AGL2005-02380/ALI and AGL2008-02135/ALI).Peer reviewe
