Samaranayake, LP

Zee, KY

Journal of Dental Research

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Title Salivary immunoglobulin A levels in "rapid" and "slow" plaqueformersAuthor(s) Zee, KY; Samaranayake, LPCitationThe 12th Annual Meeting of the International Association forDental Research (Southeast Asian Division), Phuket, Thailand, 9-12 September 1997. In Journal of Dental Research, 1998, v. 77 n.5, p. 1352, abstract no. O-12Issued Date 1998URL http://hdl.handle.net/10722/53962Rights Creative Commons: Attribution 3.0 Hong Kong License1352 Divisional Abstracts: Souitheast Asian Division J Dent Res 77 (5) 19980-12 iunnglbui A levels in "rapid' anid 'slow" plaque formene. KY. ZEE* 0- 3 Astinc,p'i W'nt of NrJli?I* nsug 5AiVsival.5~o0-12nd LP. SA ARANYAKE(Faculty of Dentistry, T'he University of Hong Kong). 0OnB.Ypga, No slK0p (a.of Denisitry,Fo.fSalivary inumunoglobulinA (s-IgA) is knowntoplay arole in aggregation oforal bacteria, and hsence, Scierse, Psisar of Sengkla Uhiiversity, Hot Yak, SonMgkla 90112, ThalandL)may affect the rate of plaque formation. Therefore, the aim of this study was to investigate s-IgA Man m l.d .havpesb inused io idensateeify aa it sasoiesi ayas maD.gNgiAl inoarlevels in "rapid" and 'slow" plaque fomrsnr. Forty-nine healthy volunteer were screened for their Honwever, inuaopluaasys still qar to lhae poeudis in the s imollogled disgasisi Ofplaque formation rate. All the subjects received oral hygiene instuctions and prophylaxis once a piootldam.la aoeo wsuywnt netgt .gniai ue mlraweek for 3 weeks in orderto upgrade their gingival health. T'hereafter, a 3-day no oral hygien erol'frm sin 1- ai W e bltig P. gasgivafiu ATCC 33277 wasstarted in order to assess the plaque foematson rate using the plaque usdex (PI). PI on the buccal uusstyuaqte saalaiiigalslnediigthM1H!,p4..00 usurfacso of all the canines, premnolars and 1st molars were recorded and 5 subjcts with the highest d15 . isute. iar a msatastiir hsotle. sesooletsdin liaMHPoleSe pHe4sdisslve inienca PI and S with the lowest mean PI were selected as "rapid" and "slow" plaque forimer, respec- osou.'a ue nnawrsa sltdb mmhgtm ti0OOghtively. Unstimulated whole asiva seas collected into a ice-chilled beaker from each of these 10 nib' the psoycienmsi utilude agam s P.wguialzuw wase soLedinrby t sn atar00pussatukw 2jects. The saliva samples were then centrifuged at Il0,000g for 10 min. and the supernsatants we outePblyceml sfraoeiosCbinsetmiP ogmgdnadimatoacy o tlrebbrste pm oueia wuerus orstored at -70"C until analysis. References curvesfors-lgA were established by testing serial dilutions bywSD-PA-IOEu AfmadWsen blmot nayis. Hve iaar guoctyiaofowxuster pmunemim gingshWumof human IgA with known concentrations. The levels of s-IgA in the saliva of all the subjects we 44t 78 kDa wecre sopised by sera raised againto Owa supanstat Mmar .kDa proteinassessied by usingaconventional ELISA technique. Differences betweenthe 2 groups were compared exhibited obvious reaotin in both supersntaw m outer-- im a -r frastionar liar spaeihetyby using Student t test. Results showed a statistically significant (p'Z0.05) difference in the mean of antibodics was ecunfimned by positive results in indirect iriurnmhsioraoeaare against P.s-lgA concentration between the "rapid" (9g±gml * 1.9) and "slow" (I 7lsg/ml * 6.3) plaque formers. *Misuts urasa thatte Outrralra f P gsragiVAlis moiaraiaiThis sgests tht SAlA may contribute tothe differencs in fth rafte ofRlague formation in these two 8*5J5i ninva e ea iga014 Effec of Dexamtliasoneon Periodontal Heaing of Replanted Dog Teeth. V.SAE- Dental and periodontal health status in Thai vegetarians.LhA', Z. METZGER, hiTROPE (University ofNorth Carolina at Chapel HEil, USA). A ANIIIOG n .PESRNRNTPrevious studes have ashown ViaSpan to bean excellent medias for the extended storage of avulsed (Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand)testis Also, it has been shown that replantation of teeth into a 48-hour-old socket resulte in a It han been found that the diet of vegetarian is beneficial to oral health, as reflected by a highpwrticulary high degree of inflammatory root resoption. It was hypotheized doat this resofption wa number of teeth present, improved caries status, and increased the resistance of periodontalrelated to M'OM 'bi withinthe tooth soeket at thius 48hour time period, reslting in a tissues. The aim of this study was to compare the dental and periodontal health status inplethor of eytolacias and other prodbuct iplicated in bone/root resorption Steroids have been vegetarians with their non-vegetasian controls, Subjects were 130 Thai lactovegetarians whoshown to iunhbt macrpsage activation and its ceeucn.The purpose of the presen maintained at leat 5 years in vegetarian diet and 130 volunteer non-vegetarian controls. Allinvestiption was to evaluate histologically, the effiect of deaethasone in the prevention of r00 subjects were interviewed about their dietary habit. Of tise lactovegetarians, 76.9%1/ had 5-14resoesionin soredandepissteddogs sees. Tentynineroot of beale dSS~years of vegetarianism and the majority (73,8%/1) ate only one meal a day. The dental andweeeta tedan ranomly assigedqto rusfr4 orsotorg.Goup1.Teeth(fgn.1) seth periodontal health status were assessed by oral examination. DMFT and gingival index werestoedr n val cotaivngViSpa ony.Gropg2Teeh n-t) wrestoed.nGvalocotaiin reistredhTe vgetrias ndwontolsdionodifeiintheriarisspevaenet(8.5 vViapa and deaehaoe(topical treatment, -ceentratod 16 ug/snlGroup 3. Teeth (n-6)' 60.8%; p>0.05). T'he number of rmaidning teeth, DMCFT and gingival index in thestoredinvials ootasmingVanSunonly,bust d amet-hason ws admnit ed _vegetarians and controls were not differet (p>0.05). Although the prevalence of periodontal(Systemnic treatmlent, 0.3mg/kg bse) 2 days prior lo, on the day of and the following 2 altenate day diseases were numerically high in both group, the vegetarians showed significantly lowerafter eVtAeto/elatto After 12 weeks, the dop were sacrificed, the teeth histlogicaly periodontal disease prevalence than the controls (81,5% vs 95.4%; p-.0008). There were noprepare man evaluated according to Andremsn The mean pereentage complete healing in the differences in DMFT and gingival index among the lactovegetarians regarding to theirGroup 2(85%) was signficantylhighertdon that in Group 1 (69%) and Goup 3 (67%). Accordingly, duration of vegetarian diet This study diboemntaehtter deta health in vegetarianthe -m percentaep inlmaoy anrplacement resoption of Group 2 (13%, 30%) were lower incmarison with their controls hut it did show lespeaece of peridodntal_disease__ntha that in Group 1 (22%. 7%) and Group 3 (28V% 5%). There was an indication that topical use of the vegeta.rianss, This study was supported by the Dental Association of Thailand.dexamethasone resulte in an increase ofcomolt healing and less resorotion cmolications.0-16 VARDuMUM GMUivnty. mstk bed 0-17 M.G. Boteho Prince PhlpD WHoapkAt Hong KongTo dsmisiina dir Who in praisdosmnbaldlicatlodaily self-adtidmi ree easwoweigaiane drevire (Wwa Pik). 7%kny Recas atudieshaveiveiogtedthe sisiirobisldhot of-snoparat antbactrial agontainto dentaludbj,s at fidly banded cidirdorsi puabrna weve miicid and smopied sotwse ur,. They bed minus slats wsntz materiaLs The addkie of antbacteral agnt ino GIC's may be usefu ma reatosng cavkiti whaer~ia5ivslr, -Sum ty diainat ysiasc usubtis iuininsin sen..r ty ascariou.,tmstin eanyarbestarnrd-ceriu yam m tmnsmdtrHiv*ordet,et*addition h oddtim ac5-iaS ivid.a ft mydim eruAc iandiims did-m we rtbo es, streWonos-i agentslos many hav datrimanstal offesa on the physial propertie ofthe reatoetive material.Thesm ofStssusssyo apsi. Stopo a o w"d i ot4o ubmwngv wrgwoltso cm this pilot study seas to invesptigt the effects en the 24 hour coaVsepea e stregths of antlsactral(ownm iioAreadtiig ironic). Sutp are wn dim dwiing in stroad aostb, abebii wein wivm sunrcions for mting din W sem agnsaa oprated intotlsaposeder of Fopi DC. Two anoawtwla agema daloohexdinehyreoidPit aissu ewd dirone iaftnidihsmtig nednics nisoino war ewfrid twoasd os--hint adamws.' ret dauly dir breakfuat (CXH) mad ruthisaide (CE'I)wvre added at 0% (eontsel)1% 3% and 6% ceaeneraion bywei&h ofad ofm uppw. mption u emd n accodance w do nudectons amewu with othiosinvailaotphspos fun ofPGPCMatIwiataw ers mixed.Maocordwhmgndaccordig ctasrurs' instructions mlMad iiseypm biaasi ns er torinae e,i i ~in5i'~ s,iisis md in~~ ~~90 cyhuhia qsecin 6nsa X 3zoma mawinswewuroiedssin a 3.0:1 powedrto ESquid ratio at 80-80%cmesuesse watrm, and kips in device ri clas is tin wedi bo ust sachise ins Ni idir firm at iris trygin swes Isa ity mad steal for 24 haour in deionizd seatr The epsmamswrvmo deetedo a oossyreiveasswee. All mbjrcu sure us si ha instei sat Wonrdt mpdiykni dam. drirgs in 5- web - oatin any. force to huimr in an lastren machine (meodellill) at a craWread apsed lmasti mad the forc inSearinsg in dine a hrIre 4 -ad 8-sushi, weid, sm -in take atOinoms- at debas, insists, plsqwt sn din MPa's caloulated. ANOVA tea showed highl tigpflan reductions in coa m re trngthlofat dlssntnis iuns papdlsy, nirsisa, ad siabawd gsgsva. Blaring index sad hawield is sodS tis-e,hud (P-0O0O01) with the addition of ofboth CET sad CXA to Fuj i[L Denatts teat showed significantOm ngkurcd. Pbqw kw Wkr orW unplar.Mw cb(Pi Ootdodedferonceaba1tweinfthe anttwaceerealtteatt grogpspanddtheecontroobaewinsl petioedosia heddihdir i 4 wria f wo winrneiigafis Vrmp (Pr 0.05). tgi*vlainianime isirporriti 3%=127MPa SD3*15.2, 6%-- 89MPa S1)13.9, CXH 1%-- I94MPa SD. 18.5,3%-- 165MPa SD* 30,slcammasd. Dsia ts o weiatienoarous-d. h si ~ernIeois su sselal iaei sreemlg 6%-Ifl0MPa SD+ 13.7.ptasr in isgcki m5emad iiltsti iAiis lb, wma ssaisdsino45penisi = ar sfias Fromthipiot study, it em be eooehsdethat h diino mlsgra aetso Pj IX shoul head. is bans, is gis5loiss as5 ia sihnclsg in s-~~~oa1 i~pie parioto avoid ei5eas an pis eLGranttCRCGG373-252-0009Isdluener if lctic mid in sufns aisrotradlema sif resu.modifasdsSia woomer reoav _ Thermocyclung assameans of inducing mechanical ftiguie in dental composites. NH Abs0-1 stleai M. NOORt ad C.G TOOt (Depsesorit if Conievsaive Dereitey. Faculy of 0- 9 Kasim* and JF McCabe. Dental Facslty, University of Malaya, Malsysia aid Dna0- 8 Den"rytiUivertyofMolaya, Kuala LiAurif, MALAYSIA) School, Usiversity of Newcastle spot Tyne, UK.Resin-mod1ilied gasim oacstcrresoraive mate"iamas becoming iouoeasingy popuAwars rrstorative mtateials becoaseif The effect of thermocycling oi the fati ue behaviour of S commercially available dental composites;thai lasroved bstdtig pupserty bust Sse in tanown insi dine aesices prpegoseuichas n siefate nicebmwdas. Tbe P50(P)l, Silux PlusS), HeliomoioeH) , Clearfit Photo Posterior-tight activated(CLA) and Cleartiletsnwof tes ustiy was to evitkats the uaifae maicmstdnrn of 4 essaciatldy avainlbt reuia-mdle OMas Posterior-chemically activated(CC) was investigated. One hundred and ten rectangular bar specimensisuse esoateeive imtwseei (Fmsj It LC (improvedt'. Phsotac.Pil'i, Virinne ind Dyemct4), a conventional Slin were prepared for each material and divided into I I groups of 10 each. Test groups 1-6 were ssbjectediointinr re5Unki5ve mtittrmi (Fui aX GPt) andsaiepoaite maerain (TPHt`) wenestored il 0.01 itoiL/ la5i5 scid. to t. 250, 500, 750, 1000 and 10000 thermal cycles respectively. Each thermal cycle consisted ofsodiuma ceste lauffee sobiion (pH 4. 1) fbr 1, 7. and 28 days as coesswaed to the asefae marondatsu onmodnitely niinuts immersion time of SO seconds per change. The control groups 7.11 were stored is distilledafler cure.wtra 7Cfrteeuvln iet opee20 0,70 00ad100temlcceTeway ssimiplofoa.cimatmiweere nditsadforeacispenod of sonvnean d 10 ofthers-mpesbetigsmugiedwiwtrt hao teeliaettmet opee20 50 5,10 n 100temlccebonfadl p n(Proinad').EactampWof10lo mon war topshkidankplsic coatiAMad storest bwuowrespectively. All specimens were subjected to a 3 point bend test and loaded at a crosshead speed ofsan at 37C ± ?C. Measrnnase waem naken at 23aC t InC by using a Sldadzudi Jimbaordmess Tager HMV-2000 mnm/mun on an Inston Testiig Machine. The flexumel streegth of all materials decreases with thewinb a Vickws- i5-emd ad tOOgma load her IS seconds. The waface of each Ipe wasme groses wih a to0o gris number of cycles and length of waler storage except for CC where the flexural strength increases dsriigcabide pager bathes 3 owiintaiioe wer inside. DMataemstlysml by uigq ANOVA ad Nlewmai_Kta-idi Mulniple abe earlier stages of thermocycling and water storage. The lowest flexural strength was observed in

Salivary immunoglobulin A levels in "rapid" and "slow" plaque formers

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Salivary immunoglobulin A levels in "rapid" and "slow" plaque formers

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