Lyamuya, EF

Matee, MI

Mesengi, AE

Samaranayake, LP

Scheutz, F

Simon, E

Journal of Dental Research

HKU Scholars Hub

Title Biotypes of oral Candida albicans isolates in a Tanzanian childpopulationAuthor(s) Samaranayake, LP; Matee, MI; Scheutz, F; Simon, E; Lyamuya,EF; Mesengi, AECitationThe 11th Annual Scientific Meeting of the InternationalAssociation for Dental Research (Southeast Asian Division),Jakarta, Indonesia, 4-5 October 1996. In Journal of DentalResearch, 1997, v. 76 n. 5, p. 1217, abstract no. 67Issued Date 1997URL http://hdl.handle.net/10722/53741Rights Creative Commons: Attribution 3.0 Hong Kong LicenseI Dent Res 76 (5) 1997 Divisional Abstracts: Sotutheast Asian Division 1217Cytotoxicity of Polyester to Hussman Cultured Lymphocytes.63 w. SISWOM lHARDJO, HARSTNI and S. SUNARINTYAS (Gadjah-Mada University, Yogyakarta, Indonesia).Acrylic resin has been the only polymeric denture base material. According to Tsuchiyact al. (J Prosth Dent 71: 618-630, 1994), residual monomer (polymethyl methacrylate)elicits iritation of the oral mucous. Siswonihardjo (preliminary study, 1994) states thatpolyester, a polymeric maesrial for staues, can be manipulated to denture base. Thisstudy examined the cytotoxicity of polyesters to cultured human lynphocytes Specimenof polyesters soaked in human saliva for 24 hours during a period of 7 days. Sevendishes of culturod human lympisocytos were treated with S % human saliva (polyesters'soaking solution), and one dish as control. After 24 hours incubation, the number ofliving lymphocytes were counted by a haenacytometer. Data analyzed by tho Anovashowed no significant influence of soaking period on tho cytotoxicity of polyesterst(,-A1 A Tot .-,n he. en.,Ml,l thO,t in a aeierind of7 davs. nolvegters denture base will notcausO toxic effiet.1-The erface between Affected Dentine and Resin-Modified Glass-iosoner.65H.K. YIP* (Department of Conservative Dentistry, Faculty of Dentistry, The Universityof Hottg Kong, fHf).Dndine remaining after chemomeclanical caries removal by N-mosnochloro-D,L-2-arninobutyric acid(NMAB) and urea containing NMAB (NMAB-Urea) was considered sound by clinical criteria. Studiesusing scanning electron microscope and electron probe microanalysis have showed that the cavity floor,i.e. affected destine, after complete caries rensoval to be of similar mineral content as the underlyingsound dentine (Caries Res 1995;29:1 11-117). The aim of this study is so use the confocal laser scanningniicroscopy (CLSM) so study the bonding interface between affccted dentine and resin-modified glass-iononcer. Carious denine was removed chemomechanically from carious lesions by means of NMABand NMAB-Urea. Teeth in which complete caries removal was deemed to have been achieved by normalcriteria were restored widt resin-modified glass-ioniomer (Vitremer). Fluorescent dye (Rbodamine B) wasincorporated into the primer and applied onto the cavities according to masufaettwer's instruction priorto rcstoration. A control cavity of the sanme depth as the carious lesion was prepared using the roaryinstrumnt on the opposite side of the solution-treated cavity-.All the control cavities were restored withcomposite (ZIOO). Black sections and thin sections (100-120gan) were prepared from the specimess tcethand exmitadl under scamintg electron microscope (SEa) and confocal laser scanning microscope(CLSM). The superficial layer of dentine remaining on the cavity floors appeared to have a vcry unevenwith many undermined areas. The primer of the resin-modified glass-ionomer penetrated non-uniformlyinto dentinal tubules of the affected dentine while the patern of penetration was much more uniform incontrol cavities. T'he results indicated that the bonding interface between resin-modiie &la oand affected dentine was differcnt from tht of sound dent'e.i1Biorypes of Oral Candida albicans Isolates in a Tanzanian Child Population L.P.67 SAMARANAYAK.E", Mt1. MATEE', P. SCHEUTZ3, E. Simos` & E.F.LYAMUYA'(tUniversity ofHong Kong and Faculty of Helath Sciences, Muhimbili, Tanzania'and Aarhus, Denmark').Although biotypes of Candida albicans from adult populations, especially in the West, havebeen described, there are no data either from a child population, or from the African continent.Hence a total of 200 oral C. albicans isolates from Tanzanian children aged 6-24 months. werebiotyped using two commercially available API micromethod kit systems and a boric-acidresistance test (Williamson, Samaranayake & MacFarlane. Mcrobios 51:159-167, 1987). Thepredominant biotypes, which comprised two thirds of the organisms isolated were, J1S (19.5%). AIS(16.0 %), JIR (14.5 %), AIR (9.5 %) and PIR (7.5 %). In total 16 new biotypescomprising 44 (22 %) isolates, which have not been described hithertofore were found in thisTanzanian population, and of these, PIR biotype predominated with 15 (7.5 %) isolates. Therewas no significant association between predominant biotypes (with clusters > 15 isolates) andthe age, gender, breast feeding. and malnutrition. These data together with our previous studiesin healthy adults from China (Xu & Samaranayake, Arch Oral Biot 40: 577-579, 1995). andHIV-infected individuals from Hong Kong, UK. Germany and Australia (Tsang ct. al. J OralPathof Mcd 24: 32-36, 1995) indicate i) biotvpe profile of C. albicans isolaies may differ in2aediatric and adult pooulations, and ii) global distribution of various sub-tpes of thismnoporrunist pathoser.Funded by Rearsrch Grants Cour.ci of Hong Kong+69 Effect of Mouthwash on Bacteremia Cases of S. virdans Post Exlracton.DJAIS. A.' ( Denta Faculty University of Indonesia ,Jakarta,lndonesia)Bactesal endocardWts caused by S. tdans bacteremia post extraction has been reported. Theeffect of 0,1% Hexeidine msoulhwash on S. sesdans bacternia post 3rd molar salpostionextraction was exarined in this study. Subjects' citeria were; 20 25 yrs; OHI-S c 2; gingivitis (-);penodontitis (-); not under astibiotic treasnst Yd molar castes free and vital. Twenty six adultswere divided randomly ints 2 groups of 13 each.The control group rinsed with saline soluton for 30seconds.The experimenl group rnsed wih 0,1% HexeWdine for 30 seconds. Third molarextractions were done In stie condition and required no longer than 3 minutes. To Identity S.viridans, samples were taken from plaque and blqod. Plaque was taken by scalers and pooled intwo starter broth media, each incubatd in aerobic and anaerobic conditions. Blood was drawn 5minutes before extraction this was folowed with blood drawn 5 ninutes and 10 minutes postextraction. One cc of blood from each draw was pooled in BHI broth and incubated aerobicaliy.FRe cc of blood-from each draw was pooled in 'RocWe blood cuturs and incubated anaoically .The resutts showed that S. vndas bactrennia 5' post extraction from contrl group was 11113eases and experintal group was 5U13 cases. S.wrdans bactemia 10 post extraction fromoonol gtoup was 8/13 cas and expennent group was 3/13 cases This resul statsticallywere signigeant dfferent (p < 0,05). te conclusion 0.1% Hextirine was slonialfant to reduce S.vidans bateremlainiaInelcd soososn.Water Storage ETmect on Diametral Teiisile Stresigth of Glass lononmers.64 D. IRNAWATI (Gadjah Mada l.Iniversity, Yogyakarta, Isidonesia).The objeclive of this study was to mcasure the diametral teiitilc strength (DTS) of resin-modified glass ionomer (RMGI) and conventional glass ionomer (G0) restOrativematerials af&er stored in wvater over time. Eighty disk (6x3 mm) samples werc made oflonositFil (RMGI) and AlpIaFil (GI). The RMGI was photocured for 40 seconds. Thesamples werc stored in distilled water at 370C for I day, 1 week, 4 weeks, 8 veeks and12 wocks. The DTS was measured by Universal Testing Maclhine at crosshead speed of0.5 mm/min. The Isean and standard deviation ofDTS (kg/Cm2) were:Water Storage lonositFil AlphaFilI day 119.4 ± 7.2 72.8 ± 10.51 weel- 119.6 ± 6.9 79.4 ± 11.24weeks 128.2 ± 13.5 105.5 ± 7.7Sweeks 138.5 ± 18.0 108.4 ± 8.712 weeks 173.1 ± 10.1 111.3 ± 16.4Anova and I-ISD-test were done. The RMGI lIad lhigher DTS thuat G1 (p0.01). TheDTS of RMGI increased from 8 weeks to 12 wocks and GI frosis I week to 4 weeks(p<0).O). In coiielusioii the glass ionomers did not show any loss after storod indistilled warut 12 weeks.Biogical evaluation on gas ionoener cement using fissue culure. J. A66 GUNAWAN- (Faculty of Dentisty, Trisaldi University, Jalerta, IndonesIa).The use of materials endodontic treatment must consider the biological effects of materials onthe denial pulp. Evenitough It has been recommended as a filling ining material, using it asa sealer is not populary kxmwm. That is why this resarch has been conducted to find out thediffemrne between the iocompatiblity effect of glss lonomer materials and the calciumhydroxide mauterial by tissue culture metod using B-K 21 (C-13) cell lines. Twenty-eighspecimen of gass iononer material and calcium hydroxide materials were divided into 2groups and control group. These specimens were put into 24 wells culture tube and BHK 21(C-13) cells were added into each well. These cells were harvested after 24 hours and thetoxic effects were observed by couing the dead cells compared with the living cells. Thet-est analysis showed that there was no significant difference (p > 0.05) between the blocom-patiblty value of glass ionomer mfIat group (92,7410 + 4,55%) and calium hydroxidematerial group (94.3090 + 2,9475). It can be cor_ded that the bilI e o t classlonomer ement was mr to that obtained in the calcium hudroxIde cement bv tissueculture, Tis findino suaoese that the tested alass lonomer cement can be used to reolacecalciumh_ roxide cenest an a saler for obturted roos.he Prevalence, -Distribution and Identification of Oral Candida in68 Smokeer. K. ITRATANA*,. P. PIPATANAGOIT and V. AIWSK5LK. Dept. OralMod. Chula. tniv. Bankok, THAILAND.the aims of this study were to determine the prevalence, distriustion andidentification of oral Candida in 47 non-smokers and 64 smokers by usingimprint culture for the isolation. The identification of Cweidia was carriedout according to Sc. Donis sod neks and also the modified criteria ofKreger-van Rij for finalAidentificatiort. The prevalence of Caradida in stoker185 percent) was significantly higher than in non-smoker (48.44 percent)(p < 0.001). lThe aucosal site fros which Cardida was most often isolatedwas the posterior dorsal.tongue and the floor of moth was the least one in bothgroups. C. lahica:n., C. oDrapsilosis and C. trooicalis were mostly identified ineach groups. The highest isolation in non-smoker was C. albicans (69.56 %tw4ereas in smoker, C. paraostlosis was fdund mostly (60.78 %M. In smoker, aoreCaidida species were identified than in non-smoker. ..It can be concluded thattobacco-swrokina influens the oral Candidal carrier rate. tore C:ar.didalspecies were found in smoker. This study was sypported by Tantaraksa ResearchFouQdation, Thailad.70 1Ftinctional Rethabilitation by Metal Reconstruction osf Post perative MandiblesH. SOPRAPt'1t0 * QProsthodontic Dept., Airiangga University, Indonesia)One of the choices in the ureatent of mandibular bone Amcloblastoma is mandibular resec-tion. In such a case, rehabilitation/reconstuction is expected to restore the mandible functions,whicbl include kincsiology, masticatory, phonetics and aesthetics. One alternative device to beused for mandibular rchabilitatioa/reconstruction is individual metal framewtork made ofCobalt Chromium casting.The present research is based on 16 clinical cases consisting of 8 cases of hemimandibiliectomyand 4 cases of partial mandibulectomy, all of which were rehabilitated by means of individualmetal framework made by the researcher, and 4 cases of eiemimandibulectomy using factorymade metal framework as a control group. After the rehabilitation, we gave a regular trainingprogram and conducted clinical sesting on the mandibular functions using 14 variables. Fromthe 14 variables tested, there was one which showed a staMlcally significant diflerence. 'thisparticular variable is Chewable foods. AU the other variables proved to be homogenous.tI'he rehabiliAti cIj of themadible Sing idividual metaLf.aMswork madle ofCobalt Chromium ma restre bula fuctions at l_astas ywell as doesshi_.actorymade framework_In far by using individual metal fmework made aoLCestaChromiumcsingubec can chew Ih foo be_I(P.-V.ul). it can DC con-guacu tuat im a-LMIVU La I URXD. w-jx-VJA U%.2stuA. ..-.

Biotypes of oral Candida albicans isolates in a Tanzanian child population

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Biotypes of oral Candida albicans isolates in a Tanzanian child population

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