Li, HM

Tsao, GSW

Wang, XH

Wong, YC

Hong Kong Medical Journal

HKU Scholars Hub

Title Functional study of expression of the Epstein Barr virusencoded LMP1 protein in nasopharyngeal epithelial cellsAuthor(s) Tsao, GSW; Li, HM; Wang, XH; Wong, YCCitationThe 8th Medical Research Conference Abstracts, Hong KongMedical Journal, Hong Kong, China, 25-26 January 2003, v. 9 n. 1Supp 1, p. 17Issued Date 2003URL http://hdl.handle.net/10722/45664Rights Creative Commons: Attribution 3.0 Hong Kong LicenseHKMJ Vol 9 No 1 February 2003 Supplement      17Functional study of expression of the Epstein Barr virus encoded LMP1 proteinin nasopharyngeal epithelial cellsA/Prof GSW Tsao, HM Li, XH Wang, YC Wong. Dept. of Anatomy, University of Hong Kong, Hong Kong, Hong Kong.Epstein Barr virus (EBV) infection has long been postulated to play a crucial role in the pathogenesis of nasopha-ryngeal carcinoma. EBV infection is an early event in nasopharyngeal carcinoma. The EBV encoded LMP1 proteinis expressed during the latent infection of the virus and is commonly detected in nasopharyngeal carcinoma cells.The pathological role of LMP1 in nasopharyngeal epithelial cells remains elusive due to the lack of cell modelrepresentative of nasopharyngeal epitheliulm. We have recently established an immortalized cell model from non-malignant nasopharyngeal epithelium. The immortalized nasopharyngeal epithelial cell line is non-tumorigenicand retains the differention properties of normal nasopharyngeal epithelial cells. This newly established nasopha-ryngeal epithelial cell system has been used to examine the functional property of LMP1 expression. Expression ofLMP1 induced agar independent growth (hallmark of malignant transformation in vitro), stimulated growth anddownregulated expression of the p16 gene in the immortalized nasopharyngeal epithelial cells. Other biologicalproperties of LMP1 expression are: increased saturation density, downregulated E-cadherin expression, increasedcell mobility and invasive property in nasopharyngeal epithelial cells. Gene expression profiling of the LMP1expressing cells reveals upregulation of genes for cell survival, angiogenesis and metastasis. Our results suggestthat LMP1 expression play an important role in promoting the conversion of premalignant nasopharyngeal epi-thelial cells to invasive cancer cells.PL5

Functional study of expression of the Epstein Barr virus encoded LMP1 protein in nasopharyngeal epithelial cells

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Functional study of expression of the Epstein Barr virus encoded LMP1 protein in nasopharyngeal epithelial cells

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