Hansen, Lisbeth Truelstrup

Hayward , Jennifer

Jackson, Amy

Jamieson, Rob

Yost, Christopher

In the Canadian Arctic, municipal wastewater is commonly discharged into tundra wetlands, where ancillary treatment has been observed to occur. Antibiotic resistant bacteria and the antibiotic resistance genes (ARGs) they contain can be present in municipal wastewater and there is limited knowledge on ARGs in wastewater in arctic environments. The objective was to assess the fate of ARGs in arctic tundra wetland ecosystems impacted by municipal wastewater sources. The two wetlands were located in the Inuit communities of Sanikiluaq (56°33’N, 079°13’W) and Naujaat (66°31’N, 086°14’W) in Nunavut, Canada. Genomic DNA was extracted from both soil and water during the spring freshet and late summer in the wetlands, and a suite of nine clinically relevant ARGs (sul1, sul2, mecA, vanA, qnrS, ermB, tetO, blaTEM, blaCTX-M), and an integron gene (int1) were analyzed using quantitative polymerase chain reaction (qPCR). Hydrological and water quality measurements were also conducted. Gene targets were consistently present in the wastewater, and throughout both wetlands, with the exception of vanA and mecA. ARGs were higher during the spring freshet, as a result of short hydraulic retention times (HRTs) (&lt;2 days). ARG concentrations at the marine receiving environments returned to near baseline in Sanikiluaq; however, elevated concentrations persisted in Naujaat. Elevated ARGs in wetland soils suggested that they may act as a long term storage reservoir for ARGs. Hydrological conditions had a large impact on the spatial distribution and levels of ARGs in the arctic environment

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Downloaded from orbit.dtu.dk on: Jul 07, 2018Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receivingmunicipal wastewaterHayward , Jennifer ; Jackson, Amy; Yost, Christopher; Hansen, Lisbeth Truelstrup; Jamieson, RobPublication date:2018Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Hayward , J., Jackson, A., Yost, C., Hansen, L. T., & Jamieson, R. (2018). Fate and transport of antibioticresistance genes in Arctic tundra wetlands receiving municipal wastewater. Abstract from 2th UArctic Congress ,.Congress - Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receiving municipal wastewaterhttps://congress.uarctic.org/program/science-section/sessions/1-environmental-protection/session-36100/abstract-38563/[27-06-2018 10:12:52]EN | RU  SearchUARCTIC EDUCATION RESEARCH MEMBERS NEWS  ABOUT  PROGRAM  PARTICIPATIONHome >  Program >  Science Section >  Sessions >  1. Environmentalprotection >  1.5 Sanitation in small Arctic communities >  Fate and transportof antibiotic resistance genes in Arctic tundra wetlands receiving municipalwastewaterFate and transport of antibioticresistance genes in Arctic tundrawetlands receiving municipalwastewaterTheme 1. Environmental protectionSessionName1.5 Sanitation in small Arctic communitiesDatetime Sep 05, 2018 02:50 PM - 03:00 PM (UTC +3)PresentationTypeOralPresenterAuthor(s) Jennifer Hayward (Dalhousie University, Canada), AmyJackson (Dalhousie University, Canada), Christopher Yost(University of Regina, Canada), Lisbeth Truelstrup-Hansen(Technical University of Denmark, Denmark), Rob Jamieson(Dalhousie University, Canada)AbstracttextIn the Canadian Arctic, municipal wastewater is commonlydischarged into tundra wetlands, where ancillary treatmenthas been observed to occur. Antibiotic resistant bacteria andthe antibiotic resistance genes (ARGs) they contain can bepresent in municipal wastewater and there is limitedknowledge on ARGs in wastewater in arctic environments.The objective was to assess the fate of ARGs in arctic tundrawetland ecosystems impacted by municipal wastewatersources. The two wetlands were located in the Inuitcommunities of Sanikiluaq (56°33’N, 079°13’W) and Naujaat(66°31’N, 086°14’W) in Nunavut, Canada. Genomic DNA wasextracted from both soil and water during the spring freshetProgramProgram OverviewMeeting SectionScience SectionSessions1. Environmentalprotection1.1 Agriculturalstructures in Arcticregions: presentfunctions, limitationsand solutions for futuresustainable connectivityin Arctic regions1.2 Internationalconservation law andlocal communities. Canlocal interests beadequately integrated?1.3 Food security andOne Healthperspectives1.4 Sustainabledevelopment of theArctic - boreal regions1.5 Sanitation in smallArctic communitiesProcess options fora sewage lagoon inArctic CanadaChallengesassociated withmechanicalwastewater facilitiesin the CanadianArcticSewage treatmentimprovements in theCONGRESSCongress - Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receiving municipal wastewaterhttps://congress.uarctic.org/program/science-section/sessions/1-environmental-protection/session-36100/abstract-38563/[27-06-2018 10:12:52]and late summer in the wetlands, and a suite of nine clinicallyrelevant ARGs (sul1, sul2, mecA, vanA, qnrS, ermB, tetO,blaTEM, blaCTX-M), and an integron gene (int1) wereanalyzed using quantitative polymerase chain reaction(qPCR). Hydrological and water quality measurements werealso conducted. Gene targets were consistently present in thewastewater, and throughout both wetlands, with the exceptionof vanA and mecA.  ARGs were higher during the springfreshet, as a result of short hydraulic retention times (HRTs)(<2 days). ARG concentrations at the marine receivingenvironments returned to near baseline in Sanikiluaq;however, elevated concentrations persisted in Naujaat.Elevated ARGs in wetland soils suggested that they may actas a long term storage reservoir for ARGs. Hydrologicalconditions had a large impact on the spatial distribution andlevels of ARGs in the arctic environment. Download to your calendarArctic community ofCambridge Bay,NunavutFate and transportof antibioticresistance genes inArctic tundrawetlands receivingmunicipalwastewaterBioelecrochemicalanaerobic sewagetreatment technology(BEAST) for Arcticcommunities –progress reportPotential af singlehousehold systemsin GreenlandicsettlementsClosing the loop oncircumpolarcommunity watercyclesAnthropogenicimpact on themicrobiota of soils inthe areas of isolatedpolar settlementsHydrologicalanalysis of municipalsource wateravailability inNunavut, Canadaaccounting forpopulation growth,infrastructureupgrades, andclimate change1.6 Digital Earthimpacts on pan-Eurasian researchprojects in the Arctic1.7 Arctic tourism andglobal change1.8 Modelling andcontrol of water andwastewater treatment inCongress Partners    Congress - Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receiving municipal wastewaterhttps://congress.uarctic.org/program/science-section/sessions/1-environmental-protection/session-36100/abstract-38563/[27-06-2018 10:12:52]the Arctic conditions1.9 Climate change,forests and otherecosystems health inthe Arctic region1.10 Biogeochemicalcycles in Arctic forests1.11 Reindeer &caribou in the Arcticsystem: Interactionsbetween environmental,social and biophysicalprocesses1.12 Arctic resourcegeopolitics, oil and gas -actors, policies,platforms2. 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Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receiving municipal wastewater

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Fate and transport of antibiotic resistance genes in Arctic tundra wetlands receiving municipal wastewater

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