This study aimed to generate the genetic profile of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Intan Besar
Island, Langkawi, Kedah Darul Aman, Malaysia and then to determine the phylogenetic relationship between H. leucospilota
and other members of genus Holothuria available in the GenBank using partial sequences of cytochrome c oxidase I (COI)
mitochondrial DNA (mtDNA) gene. In this study, tree topologies of neighbour joining, maximum parsimony and maximum
likelihood show that H. leucospilota has its own monophyletic clade clearly distinct from the other species and it is genetically
closer to Holothuria (Acanthotrapeza) coluber Semper 1868
