Objective
The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation – 8-isoPGF2α, and the activity of phospholipase A2 and PAF-acetylhydrolase that participate in releasing 8-isoPGF2α from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD).
Material and Methods
Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF2α were determined in the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A2 and PAF acetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) after antibiotics treatment.
Results
An almost 3-fold higher level of the total plasma 8-isoPGF2α was observed in LA patients compared to the controls, while in the urine it increased over 5-fold. The plasma PLA2 activity was more than 3-fold higher in LA patients than in the healthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total 8-isoPGF2α level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasma activity of PAF-AH in the LA patients was increased, while the cPLA2 activity decreased after antibiotics treatment.
Conclusions
It may be suggested that in the course of LA, the level of binding 8-isoPGF2α is significantly enhanced, and it may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritis unresponsive to antibiotics