Hypofractionated Versus Conventional Fractionated Radiation: A Network Analysis
Introduction: Conventional fractionated (CF) and hypofractionated (HF) are two radiotherapy methods against cancer which are applied in medicine. Understanding efficacy and molecular mechanism of two methods implies more investigations. In the present study proteomic findings about the mentioned methods relative to the controls are analyzed via network analysis.
Methods: The significant differentially expressed proteins (DEPs) of prostate cancer (PCa) cell line DU145 in response to CF and HF radiation therapy versus controls were extract from literature. The protein-protein interaction (PPI) networks were constructed via STRING database via cytoscape software. The Networks were analyzed by “NetworkAnalyzer” to determine hub DEPs.
Results: Number of 126 and 63 significant DEPs were identified for treated DU145 with CF and HF radiation respectively. The PPI networks were constructed by the queried DEPs plus 100 first neighbors. ALB, CD44, THBS1, EPCAM, F2, KRT19, and MCAM were highlighted as common hubs. VTM, OCLN, HSPB1, FLNA, AHSG, and SERPINC1 were appeared as discriminator hub between the studied cells.
Conclusion: The 70% of hubs were common between CF and HF conditions and induce radio-resistance activity in the survived cells. Six central proteins were introduced that discriminate function of the two group of irradiated cells. Based on these finding it seems that DU145-CF cells are more radio-resistant relative to the DU145-UF cells
