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Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection Of Microrna
Authors
Maxim V. Berezovski
Shahrokh M. Ghobadloo
+3 more
Nasrin Khan
Dmitry M. Kolpashchikov
Mahmoud Labib
Publication date
15 October 2013
Publisher
'Information Bulletin on Variable Stars (IBVS)'
Abstract
MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis. © 2013 American Chemical Society
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Last time updated on 18/10/2022