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Solid-Supported Synthesis Of Polymerizable Lanthanide-Ion Chelating Lipids For Protein Detection
Authors
Manas K. Haldar
Suad Nadi
Marina Santos
Publication date
7 April 2005
Publisher
'Information Bulletin on Variable Stars (IBVS)'
Abstract
Usually, lipids are synthesized employing solution-phase organic synthesis techniques. Though successful, the purifications can be difficult to accomplish due to the amphiphilic nature of the molecules. Herein, we demonstrate the advantages of a solid-phase approach for preparing a variety of metal-chelating lipids. A number of saturated and polymerizable metal-chelating lipids were prepared using this methodology. This approach requires one Chromatographic purification after cleaving the lipids from the solid support. We also demonstrate that the resulting polymerized liposomes (containing Eu 3+) possess the appropriate luminescence properties for the qualitative and quantitative determination of proteins. © 2005 American Chemical Society
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Last time updated on 19/07/2022