Blanco, null

Ibarra, null

Oteo, null

Portillo, null

Pérez-Martínez, null

Santibáñez, null

English

Portillo, null [0000-0002-2055-1825]

Santibáñez, null [0000-0002-4996-288X]

Blanco, null [0000-0002-4268-0150]

Oteo, null [0000-0001-7080-6542]

Repositorio de Universidad de La Rioja

10.1111/j.1469-0691.2008.02250.xGenetic characterisation of ompA, ompB and gltA genes from CandidatusRickettsia riojaA. Portillo, V. Ibarra, S. Santibáñez, L. Pérez-Martı́nez, J. R. Blanco and J. A. OteoÁrea de Enfermedades Infecciosas, Hospital San Pedro-Centro de Investigación Biomédica de La Rioja(CIBIR), La Rioja, SpainRickettsiosis are emerging and re-emerging infec-tions distributed all over the world. In the lastyears ‘new’ Rickettsia species have been involvedas human pathogens [1–3]. Candidatus Rickettsiarioja is a ‘new’ spotted fever group Rickettsiainvolved in Dermacentor-borne necrosis erythemaand lymphadenopathy (DEBONEL) or tick-borne-lymphadenopathy (TIBOLA) cases from Spain[4,5]. In 2006, a fragment of the ompA gene fromEDTA-treated blood of two patients who devel-oped DEBONEL after being bitten by Dermacentormarginatus in La Rioja, was submitted to GenBank(GenBank accession no. EF028201). This Rickettsiaspecies has also been amplified from ticksremoved from patients with DEBONEL.The aim of this study was to characterise thenucleotide sequence of ompA, ompB, citrate sintase(gltA) and 17 KDa genes from Candidatus R. rioja.As part of a retrospective study, 15 DNA extractsused for ompA PCR that showed 98% similaritywith Rickettsia raoultii and with uncultured Rick-ettsia sp. RpA4, DnS14 and DnS28, were furtheranalysed for the presence of ompB, gltA and17 KDa. Specimens were obtained from twoblood samples and nine D. marginatus removedfrom DEBONEL patients and from four D. mar-ginatus removed from asymptomatic people.For our 15 specimens, when ompA nucleotidesequences were compared with GenBank data,>99% similarity was found with Candidatus Rick-ettsia rioja (Fig. 1). Unfortunately, all attempts toamplify ompB and gltA genes from the two bloodsamples failed. However, PCR amplicons forompB and gltA were obtained from 12 ⁄ 13D. marginatus (92.3%). Subsequent sequence anal-ysis of ompB PCR products showed highestsimilarity (<98.9% for all cases) with the ompBgene from R. raoultii. For gltA amplicons, highestidentity was also found with R. raoultii (<99.7%for all samples). Nucleotide sequences for partialompB and gltA genes obtained in this study will besubmitted to GenBank in order to characteriserickettsial genes of Candidatus R. rioja. By usingthe primers that amplify a fragment of the 17 KDagene, we could not get any positive result.Nucleotide sequences for the ompA gene fromtwo blood samples and 13 D. marginatus showed>99% identity with Candidatus R. rioja. For theompB and gltA amplicons obtained from 12D. marginatus, highest identities for ompB andgltAwere found with partial genes from R. raoultii(<98.9% and <99.7%, respectively). These com-parisons suggest that Candidatus R. rioja can beconsidered a new Rickettsia species as soon as anestablished isolate is available.ACKNOWLEDGEMENTSFinancial support was provided in part by a grant from‘Instituto de Salud Carlos III’ (EMER 07 ⁄ 033), Ministerio deSanidad y Consumo (Spain).REFERENCES1. Oteo JA, Ibarra V, Blanco JR et al. Dermacentor-bornenecrosis erythema and lymphadenopathy: clinical andepidemiological features of a new tick-borne disease. ClinMicrobiol Infect 2004; 10: 327–331.2. Jado I, Oteo J, Aldámiz M et al. Rickettsia monacensis, a newrickettsia species causing human diseases. Emerg Infect Dis2007; 13: 1405–1407.3. Aguirrebengoa K, Portillo A, Santibáñez S, Marı́n JJ,Montejo M, Oteo JA. Human Rickettsia sibirica mongolitimo-nae infection, Spain. Emerg Infect Dis 2008; 14: 528–529.4. Ibarra V, Oteo JA, Portillo A et al. Rickettsia slovaca infection:DEBONEL ⁄TIBOLA. Ann N Y Acad Sci 2006; 1078: 206–214.5. Ibarra V, Portillo A, Santibáñez S et al. DEBONEL ⁄TIBOLA:Is Rickettsia slovaca the only etiological agent? Ann N Y AcadSci 2005; 1063: 346–348.Corresponding author and reprint requests: J. A. Oteo, Área deEnfermedades Infecciosas, Hospital San Pedro-Centro deInvestigación Biomédica de La Rioja (CIBIR), Calle Piqueras98, 26006 Logroño, La Rioja, SpainE-mail: jaoteo@riojasalud.esThe authors have declared no potential conflicts. 2009 The AuthorsJournal Compilation  2009 European Society of Clinical Microbiology and Infectious Diseases, CMI, 15 (Suppl. 2), 307–308074.510203040506070Honei Thai Slovaca U43790-africae Parkeri Sibirica U43791-Astrakhan Israeli Rickettsii BAR29 Massiliae Rhipicephali AY259083-aeschlimanniiHeilongjiangensis Heilongjiangii Japonica AY062007-amblyommiiMontana Candidatus R. rioja DnS14 Raoultii RpA4 In56Monacensis IRS3AF149108-australis Conorii Fig. 1. Phylogenetic tree with different Rickettsia species.308 Clinical Microbiology and Infection, Volume 15, Supplement 2, December 2009 2009 The AuthorsJournal Compilation  2009 European Society of Clinical Microbiology and Infectious Diseases, CMI, 15 (Suppl. 2), 307–308

Genetic characterisation of ompA, ompB and gltA genes from Candidatus Rickettsia rioja

https://investigacion.unirioja.es/documentos/5d318c8d2999521056384ca8/f/6225f2fa5fa07802f2a54cbb.pdf

Genetic characterisation of ompA, ompB and gltA genes from Candidatus Rickettsia rioja

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