Study the beneficial role of laser irradiation combination with indirect swim-up sperm preparation technique against oxidative DNA damage in infertile men
Background: In order to offer successful assisted reproductive procedures, a variety of in vitro sperm preparation techniques were created to separate normal and motile spermatozoa from other constituents of the sample. Much research on the laser as a sperm motility stimulant has been undertaken, and the results have indicated that the Laser has a good effect on sperm activation in vitro and improves progressive forward movement. Objective: This study is aimed to identify the differences in sperm activation by ISU with or without laser methods and compare them. And detection of oxidative damage to the DNA before and after laser by assessment of the 8-Hydroxydeoxyguanosine (8-OHDG) as a biomarker. Patients and Methods: The current study was conducted on 30 semen samples, divided into two groups (Asthenozoospermia and Normozoospermia individuals), during the period of attendance at the infertility clinic at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. From November 2021 until March 2022. Each sperm sample was separated into three portions
