Gleerup, David Grandjean

Larsen, Tobias Johan

Sørensen, Anna Elleby

Interessen for naturlige hygiejne produkter, såsom tandpasta, er stigende i det moderne samfund.Formålet med denne rapport er at undersøge de anticariogene egenskaber af flavonoiderne naringenin, apigenin og kaempferol. De antibakterielle egenskaber af disse flavonoider var testet på de cariogene bakterier S. mutans og S. sobrinus efter 24 og 48 timer. Siden bakterierne også danner biofilm på tænderne, hvilket gør dem sværere at behandle, blev et biofilm assay udført med brug af naringenin (500-250-125-62.5-31.25µg/mL) og apigenin (50-25-12.5-6.25-3.125µg/mL). Kun naringenin viste en hæmmende effekt. Den hæmmende effekt blev observeret i alle koncentrationer (1000-500-250-125-62.5-31.25µg/mL) på begge bakterier. Den største effekt blev set ved 500µg/mL efter 48t, med 59,56% hæmning på S. mutans og 62% på S. sobrinus. Ligeledes var 500µg/mL den mest effektive dosis i biofilm assayet, med 82,1% hæmning af biofilm dannelsen. Naringenin vise en dose-dependent effekt. Apigenin viste ingen effekt på biofilm dannelse.Disse resultater indikerer at naringenin kan blive brugt som et anticariogent produkt i tandpasta, dog har det behov for at være til stede i relativt høje koncentrationer.The interest in natural, everyday hygiene products, such as toothpaste, is increasing in the modern society.The aim of this report is to investigate the anticariogenic properties of the flavonoids naringenin, apigenin and kaempferol. The antibacterial properties of these flavonoids were tested on the cariogenic bacteria S. mutans and S. sobrinus after 24 and 48h. Since the bacteria also form biofilm on the teeth, making them harder to treat, a biofilm assay was conducted with the use of naringenin (500-250-125-62.5-31.25µg/mL) and apigenin (50-25-12.5-6.25-3.125µg/mL).Only naringenin showed an inhibitory effect. The inhibitory effect was seen in all concentrations (1000-500-250-125-62.5-31.25µg/mL) on both bacteria. The largest effect was seen at 500µg/mL after 48h, with 59.56% inhibition on S. mutans and 62% on S. sobrinus. Likewise was 500µg/mL the most effective dose in the biofilm assay, with 81.1% inhibition of biofilm formation. Naringenin showed a dose-dependent effect. Apigenin showed no effect on biofilm formation.These results indicate that naringenin could be used as an anti-cariogenic compound in toothpaste, however it needs to be present in relatively high concentrations.<br/

Can certain flavonoids be used as an anti-bacterial agent against dental caries?

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Roskilde Universitet