Diego Lijavetzky

Javier Ibáñez Marcos

José Miguel Martínez Zapater

Noelia Alañón

Nuria Mauri Panadero

Pablo Carbonell-Bejerano

Yolanda Ferradás Rial

Repositorio de Universidad de La Rioja

Oral & Poster AbstractsQuita marcas de agua WondersharePDFelement   This one (far right on the logo document seems appropriate as it provides the full name if not every one knows what ISHS  is.  Maybe we could have this on the website but on all other documentation and signature banner etc we have the second one. (also far right on the logo document.  ISGPB2021 |  31 October 2021 to 5 November 2021 |   Stellenbosch, South Africa 126P50Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotypeNoelia Alañóna*, Pablo Carbonell-Bejeranob, Nuria Mauric, Yolanda Ferradása, Diego Lijavetzkyd, José Miguel Martínez-Zapatera, Javier IbáñezaaInstituto de Ciencias de la Vid y del Vino, ICVV, Finca La Grajera, Ctra. de Burgos Km. 6 LO-20 - salida 13, 26007 Logroño, La Rioja, SpainbDepartment of Molecular Biology, Max Planck, Institute for Developmental Biology, 72076 Tübingen, GermanycCentre for Research in Agricultural Genomic, Consortium CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, 08193 Barcelona, SpaindFacultad de Ciencias Agrarias, IBAM, Instituto de Biología Agrícola de Mendoza, UNCuyo, CONICET, Almirante Brown 500, M5528AHB Chacras de Coria, Mendoza, Argentina*Presenting author: noelia.alanon@icvv.esDomesticated grapevine (Vitis vinifera L.) is used for wine, fresh fruit, raisins and juice production. Two subspecies can be identified within this species: V. vinifera ssp. vinifera, the cultivated form comprising mostly hermaphrodite and some female cultivars and V. vinifera ssp. sylvestris, the suggested wild dioecious ancestor. Studies dealing with this trait identified a major QTL on chromosome 2 as the grapevine Sex Determining Region (SDR), which harbours several proposed candidate genes. The aim of this work is the genetic and molecular characterization of a Tempranillo Tinto somatic variant that shows an androgenized flower phenotype. Whilst flowers in this somatic variant develop normal stamens, they present a reduced gynoecium that, unlike canonical male flowers of V. vinifera ssp. sylvestris, still enable fruit setting and ripening. Phenotyping results of a self-cross progeny of this variant line (more than 100 offspring) indicated that the mutant flower phenotype is inheritable. Furthermore, genotyping results of the microsatellite marker VVIB23, linked to the SDR, showed that the putative mutation co-localizes with this locus. One of the proposed female development inhibitor genes underlying the SDR locus is VviAPT3, which encodes an adenine phosphoribosyl transferase that may inactivate cytokinins by using them as substrate. The inactivation of these hormones, which promote gynoecium development in wild male vines if applied exogenously, could explain the mutant phenotype. RT-qPCR and RNA-seq expression analyses during flower development demonstrated the overexpression of VviAPT3 in the mutant line compared to a normal flower Tempranillo Tinto line used as control. Several experiments are ongoing to identify the genetic variation that causes this male-like phenotype, such as the comparison of the whole genome sequences of the variant and a control Tempranillo line, or the genotyping of VviAPT3 and other candidate genes through Sanger sequencing.Keywords: Grapevine, flower mutant, sex determinationQuita marcas de agua WondersharePDFelement

English

Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype

Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype

Abstract

Similar works

Full text

Available Versions

Repositorio de Universidad de La Rioja