Dopamine autoreceptors (DARs) and dopamine transporters (DATs) influence dopamine transmission in the brain\u27s mesolimbic pathway. Prior studies have focused on how activation of DARs influences trafficking of DATs, but how DATs influence DARs remains unclear. Male and female C57BL/6J mice received daily injections of either cocaine (DAT blocker) or saline for seven days then underwent stereotaxic surgery to obtain fixed potential amperometric recordings of DAR-mediated dopamine release. All mice received a mid-surgery injection of cocaine, and DAR-mediated dopamine release was assessed once more during maximal DAT blockade. The current study found that DAR functionality was increased during peak cocaine effects, suggesting DAR work harder to maintain homeostasis. Additionally, DAT function was increased following chronic cocaine exposure, but DAR functioning was unchanged, indicating DAR may be resistant to drug-induced alterations. Understanding the neurochemical mechanisms that control dopamine signaling is critical for informing treatment efforts for addiction, ADHD, and depression
