Analysis of prion protein coding gene polymorphisms in palestinian native sheep breeds [Filistin yerli koyun ırklarında prion protein kodlama geni polimorfizmlerinin analizi]

Abstract

2-s2.0-85077080786Prion protein coding gene (PRNP) is the genetic locus correlated with the greatest impact on classical scrapie susceptibility in sheep. At codons 136, 154, and 171 of PRNP alanine/arginine/glutamine (ARQ) and valine/arginine/glutamine (VRQ) haplotypes, in turn, are related to susceptibility to classical scrapie while alanine/arginine/arginine ARR haplotype is correlated with resistance. The aim of the present study was to genotype the Palestinian native sheep breeds for detection of genetic resistance. A total of 38 healthy sheep from Awassi and Assaf breeds were randomly sampled. Genomic DNA was isolated from blood samples. After PCR amplification and DNA sequencing, ARQ, ARR, ARH, AHQ, ARL and VRQ alleles and ARR/ARQ, ARQ/ARQ, ARQ/ARL, ARH/ARQ, ARH/ARL, AHQ/ARQ and ARQ/VRQ genotypes were detected in PRNP gene. ARQ allele was found as a predominant allele in this study with the frequency of 0.76 for Awassi and Assaf breeds while the uncommon allele ARL was identified at low frequencies in both breeds. In addition, two different polymorphisms were recognized (V12I and L23H) at different codons of PRNP. Results have indicated that most of the genotypes belong to risk group 3. The careful dissemination of ARR/ARR sheep is suggested to increase resistant allele frequencies in Assaf and Awassi breeds. © 2019, Chartered Inst. of Building Services Engineers. All rights reserved.Ege Ãœniversitesi Firat University Scientific Research Projects Management Unit, FÃœBAP: 2016-FEN-013Scientific Research Projects of Ege University with a project number 2016-FEN-013. 10th International Animal Science Conference, 26-28 October 2018, Antalya, Turkey.This study is the master thesis of the first author and was supported by Scientific Research Projects of Ege University with a project number 2016-FEN-013. The summary of this research was presented in 10th International Animal Science Conference, 26-28 October 2018, Antalya, Turkey