Not AvailableAmong 454 SSR markers, 118 markers exhibited polymorphism. Among these, three best polymorphic
markers on each chromosome covering all 12 chromosomes were employed for selective genotyping of 188
F2 mapping population derived from APMS-6B, a low stigma exsertion parent and BF-16B, a high stigma
exsertion parent and one marker on each chromosome and two reported markers used for single marker
analysis. For phenotypic study parents, F1 and F2 population were evaluated for stigma exsertion trait in
order to understand the inheritance as well as linkage with SSR markers. A co-segregation analysis with
respect to marker (based on the amplification pattern of the marker) and trait phenotype revealed that
three markers RM20060 on chromosome 6, RM5647on chromosome 8 and RM4771 on chromosome 10
showed least recombination frequency and these three markers found to be linked to the stigma exsertion
trait. These markers could be used in marker assisted selection programs to develop a male sterile line with
a high stigma exsertion.Not Availabl
