Human practices in which animals are involved often include the application of technology: some farmed animals are for example milked robotically or monitored by smart technologies, laboratory animals are adapted to specific purposes through the application of biotechnologies, and pets have their own social media accounts. Animal ethicists have raised concerns about some of these practices, but tend to assume that technologies are just neutral intermediaries in human-animal relations. This paper questions that assumption and addresses how technologies might shape human-animal relations in non-neutral ways. Building on the technological mediation approach, it proposes that technologies can influence human-animal relations by amplifying and reducing certain aspects of animals in human perception or by inviting and inhibiting certain actions towards animals. The paper next considers, in two concretizing steps, how this theoretical starting point can enrich ethical discussions on technology and human-animal relations. First, it shows how the technological mediation approach can help to conceptualize a main concern that has been raised regarding the impact of technologies on human-animal relations, namely the concern that animals might be ‘instrumentalized’ or ‘objectified’ in certain technological practices. Second, it considers how this approach can guide investigations of how particular technologies might affect human-animal relations, taking genetic selection technologies as used in livestock breeding as a case. The paper closes by briefly reviewing the prospects and challenges for the application of the technological mediation approach to human-animal relations, thus sketching directions for future research