Not AvailableParatuberculosis is an economically important, chronic, and incurable
disease in ruminants, caused by Mycobacterium avium subspecies paratuberculosis (MAP).
Understanding the genetic variability of MAP strains is important in diagnosis, epidemiological
investigation, and the formation of strategies for prevention and control of the disease.
Methods: In the present study, a total of 61 MAP isolates obtained from different parts and
species of India were typed using IS1311 polymerase chain reaction-restriction endonuclease
analysis (PCR-REA) to analyze the genetic difference(s), if any, between them and the
host adaptation.
Results: Based on PCR-REA results, bison B type was detected in 54 (87%) MAP isolates
obtained from cattle, sheep, and goats. Of these, 19 were from sheep of the Rajasthan
(n = 17) and Bareilly (n = 2), North India regions, 28 were from cattle of Chennai, South India
(n = 3), Bareilly, North India (n = 3), and Nagpur, West India (n = 22), and seven goat isolates
from Bareilly, North India region. The ‘C’ type strain was detected in only seven cattle isolates
obtained from the Bareilly region.
Conclusion: The study revealed that in India, bison B-typeMAP strainswere prevalent in most
of the ruminant species. These results have important epidemiological implications with
regard to control and prevention of paratuberculosis in India.Not Availabl
