Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Abstract
The 31 day long trial included 24 Yorkshire piglets divided into three groups. Before weaning, the piglets were given the experimental diets in a 7-day adjustment period. During this period as well as for 22 days following, the piglets were fed on diets containing 22% CP and then diets containing 20% CP for the remaining 19 days. The desired level of phosphorus in the diet for the control group and experimental group III was achieved by the addition of dicalcium phosphate, while the diet for group II was without added dicalcium phosphate. In addition the, diets for groups II and III were enriched with crude phytase (produced by growing an isolate of Aspergillus awamori) in an amount sufficient to provide 800 FU/kg. Performance and state of health were monitored during the trial and at the end the piglets were killed for collection of bone samples. Growth performance was improved by adding microbial phytase. Ash and calcium content in the tibia showed no statistical differences between the groups, while content of phosphorus was significantly increased in both groups fed added phytase in the diet. The length and width of the tibia and the intensity of its shadow indicated normal physiological processes in bone formation and ossification. The best ossification was obtained in the first experimental group, based on almost invisible nutrition channels inside the thick shadow of compacta, as well as the homogenous appearance of tibiae spongiosa in the mid diaphysis area.Ogled je izveden na 24 odbijene Jorkšir prasadi podeljenih u tri grupe, a trajao je 31 dan. U pripremnom periodu od 7 dana pre odbijanja, kao i tokom naredna 22 dana prasad su hranjena smešom sa 22% proteina, a zatim smešom sa 20% proteina do kraja ogleda. Planirani nivoi fosfora u I i III grupu postignuti su dodavanjem dikalcijum fosfata, dok su smeše za ishranu II grupe prasadi bile bez dodatog dikalcijum fosfata. Smešama za K i O-ll grupu prasadi dodata je sirova fitaza (izolovana iz gljivica Aspergillus awamori) u količini koja je obezbedila 800 FU/kg hrane. Tokom ogleda praćeni su proizvodni rezultati i zdravstveno stanje, a na kraju ogleda izvršeno je žrtvovanje prasadi u cilju uzimanja uzoraka kostiju za predviđena ispitivanja. Proizvodni rezultati su bili poboljšani korišćenjem fitaze mikrobijalnog porekla. Razlike u sadržaju pepela i kalcijuma tibije nije se statistički razlikovao, dok je sadržaj fosfora signifikantno rastao u grupama sa dodatom fitazom mikrobijalnog porekla. Rezultati morfometrijskih i radiografskih ispitivanja ukazala su na normalne fiziološke procese osifikacije kostiju. Na osnovu dobijenih rezultatanajbolje izražen proces osifikacije kostiju bio je u prvoj oglednoj grupi prasadi
