Corresponding author (NCNPR): Ahmed Elbermawi, aeelberm@olemiss.eduhttps://egrove.olemiss.edu/pharm_annual_posters_2022/1006/thumbnail.jp

Ali, Zulfiqar

Elbermawi, Ahmed

Khan, Ikhlas A.

Zulfiqar, Fazila

English

eGrove (Univ. of Mississippi)

University of Mississippi eGrove Annual Poster Session 2022 Annual Poster Session 10-11-2022 Phytochemical investigation of Striga asiatica Ahmed Elbermawi Mansoura University, aeelberm@olemiss.edu Fazila Zulfiqar University of Mississippi Ikhlas A. Khan University of Mississippi Zulfiqar Ali University of Mississippi Follow this and additional works at: https://egrove.olemiss.edu/pharm_annual_posters_2022 Recommended Citation Elbermawi, Ahmed; Zulfiqar, Fazila; Khan, Ikhlas A.; and Ali, Zulfiqar, "Phytochemical investigation of Striga asiatica" (2022). Annual Poster Session 2022. 7. https://egrove.olemiss.edu/pharm_annual_posters_2022/7 This Book is brought to you for free and open access by the Annual Poster Session at eGrove. It has been accepted for inclusion in Annual Poster Session 2022 by an authorized administrator of eGrove. For more information, please contact egrove@olemiss.edu. ❑ Striga asiatica (Scrophulariaceae) is the most widespread Strigaspecies,which has a geographic range spanning from South Africa toEast Africa and from the Arabian Peninsula to Far East Asia.❑ It is a hemiparasite and its main food sources are sorghum andmillet in India and Pakistan, maize in Cambodia, China, andThailand, Vietnam, Malaysia, Indonesia, and mainly rice inthe Philippines.❑ In sub-Saharan Africa, it is regarded as one of the most significant biotic constraints for subsistenceagriculture known as witchweed in China, which has been used as a treatment for hepatitis and irritability ininfants.❑ Previous phytochemical investigation of S. asiatica whole plant reported in isolation of apigenin, acacetin,chrysoeriol, 5-hydroxy-7, 4` -dimethoxyflavone, 5,7-dihydroxy, 3`,4`-dimethoxyflavone, and 5-hydroxy-7,3`,4` -trimethoxy flavone, two caffeic acid sugar esters, verbascoside and isoverbascoside, as well as onenorsesquiterpene, blumenol A from the whole plant.❑ This study deals with the isolation and characterization of various phytochemical constituents fromdifferent fractions of the methanolic extract of S. asiatica aerial parts.Phytochemical investigation of Striga asiaticaAhmed Elbermawi1,2, Fazila Zulfiqar2, Ikhlas A. Khan2 and Zulfiqar Ali21Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura  35516, Egypt2National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USAIntroduction Material and Method❖ Striga asiatica was collected in Hombolo, Dodoma region, Central Zone,Tanzania, parasitic on sorghum in April 2014, identity and confirmed bythe Herbarium of University of Dar es Salaam, Tanzania staff members.❖ The powdered air-dried aerial parts 150 g were extracted by macerationwith 90% MeOH at room temperature. The solvent-free extract (23.5 g)was successively fractionated with petroleum ether, ethyl acetate and n-butanol.❖ To obtain pure phytochemicals from different fractions, variouschromatographic procedures including column chromatography overnormal/reversed silica gel and Sephadex LH-20, as well as, preparativeTLC were employed.❖ NMR and mass spectra were recorded for purified compounds.AcknowledgementsGratefully, the first author acknowledges the ministry of higher education,Egypt for scholarship support..Spallek, T., Mutuku, M., & Shirasu, K. (2013). The genus S triga: a witch profile. Molecular plant pathology, 14(9), 861-869.Huang, W., Wu, S. B., Wang, Y. L., Guo, Z. Y., Kennelly, E. J., & Long, C. L. (2013). Chemical constituents from Striga asiaticaand its chemotaxonomic study. Biochemical Systematics and Ecology, 48, 100-106.Nakanishi, T., Ogaki, J., Inada, A., Murata, H., Nishi, M., Iinuma, M., & Yoneda, K. (1985). Flavonoids of Striga asiatica. Journalof natural products, 48(3), 491-493.Isolated compounds from S. asiaticaResults and Discussion❖ Thirteen compound were isolated from different fractions of the methanolicextract of S. asiatica aerial parts.❖ Structure elucidation of isolated compounds were accomplished by 1D and2D NMR, optical rotation, and ESIMS spectral data analysis.❖ Compounds 8 was found to be previously undescribed and identified as (5-hydroxy-5-(2-hydroxyethyl)cyclopent-2-ene-1,2-diyl)dimethanol.❖ Compounds 4, (pinoresinol) & 11 (mussaenoside) are for the first time tobe reported from Striga genus.Key HMBC correlations of compounds 8-9. 1H NMR Spectrum of 8 DEPTQ spectrum of 8 1H NMR (400 MHz, Methanol-d4) δ 5.66 (m, 1H, H-3), 4.12 (d, J = 2.0 Hz, 2H, H-8), 3.81 (m, 1H, H-7a), 3.74 (ddd, J = 13.7, 9.9, 5.7 Hz, 1H, H-7b), 3.66 (d, J = 4.9 Hz, 1H, H-9a), 3.54 (d, J = 4.7 Hz, 1H, H-9b), 2.50 (t, J = 5.2 Hz, 1H, H-1), 2.46(m, 1H, H-4a), 2.24 (ddt, J = 16.6, 2.9, 1.5 Hz, 1H, H-4b), 1.96 (ddd, J = 14.4, 8.1, 6.5 Hz, 1H, H-6a), 1.86 (ddd, J = 13.7, 7.9, 5.7 Hz, 1H, H-6b).13C NMR (100 MHz, MeOD) δ 144.9 (C-2), 126.6 (C-3), 83.3 (C-5), 61.6 (C-9), 61.4 (C-8), 60.4 (C-1), 60.1 (C-7), 45.9 (C-4), 39.5 (C-6).NMR of compound 8

Phytochemical investigation of Striga asiatica

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Phytochemical investigation of Striga asiatica

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